AN ENZYME IMMUNOASSAY FOR ANATOXI-A

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$68,318.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
8376
Agency Tracking Number:
8376
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9924 - W 74th St, Eden Prairie, MN, 55344
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DR RICHARD A AMOS
(612) 829-2712
Business Contact:
() -
Research Institution:
n/a
Abstract
CONSIDERABLE ATTENTION HAS BEEN FOCUSED UPON THE POSSIBLE USE OF TOXIC BIOLOGICAL AGENTS IN BOTH SOUTHEAST ASIA AND AFGHANISTAN. SUCH REPORTED INCIDENTS INDICATE A NEED FOR IMPROVING THE UNITED STATES' CAPABILITY OF ACCURATELY DETECTING A VARIETY OF NATURALLY OCCURRING TOXINS. ONE SUCH TOXIN OF CONSIDERABLE INTEREST IS ANATOXIN-A, A SECONDARY METABOLITE OF THE CYANOBACTERIA (BLUE-GREEN ALGAE) ANABENA FLOS-AQUAE, WHICH HAS BEEN SHOWN TO BE EXTRAORDINARILY TOXIC. NUMEROUS EXAMPLES OF ANIMAL DEATHS HAVE BEEN REPORTED AFTER DRINKING FROM LAKES AND PONDS FOLLOWING BLOOMS OF THIS FRESH-WATER ALGAE. WE PROPOSE TO DEVELOP AN IMMUNOASSAY TEST, BASED ON EITHER AN RIA OR AN ELISA FORMAT, THAT WILL PERMIT RAPID DETECTION OF THIS TOXIN DIRECTLY IN WATER SAMPLES. PREVIOUS SYNTHETIC WORK HAS MADE AVAILABLE AMPLE QUANTITIES OF THE TOXIN FOR TEST DEVELOPMENT AND THE SYNTHETIC ROUTE IS READILY ADAPTABLE FOR RADIOLABELING THE TOXIN. POLYCLONAL ANTIBODIES AGAINST THIS HAPTEN WILL BE RAISED USING CONVENTIONAL METHODS AND THOSE ANTIBODIES WILL BE EVALUATED FOR THEIR USE IN BOTH RIA AND ELISA FORMATS.

* information listed above is at the time of submission.

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