ENZYME IMMOBILIZATION FOR REMOVAL OF TOXIC METABOLITES

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7986
Amount: $398,370.00
Phase: Phase II
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
9924 W 74th St, Eden Prairie, MN, 55344
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
ENZYME DEFICIENCIES ASSOCIATED WITH INBORN METABOLIC DEFECTSARE USUALLY FATAL WITHIN THE FIRST FEW YEARS OF LIFE. TREATMENTS SUCH AS DIET MANIPULATION, GENE REPLACEMENT THERAPY, CHEMOTHERAPY, TRANSFUSIONS, TRANSPLANTATIONS, AND PERITONEAL DIALYSIS HAVE PRODUCED MARGINAL IMPROVEMENTS IN THESE PATIENTS. ENZYME REPLACEMENT THERAPY IS AN APPEALING ALTERNATIVE OR ADJUNCT TO THESE TREATMENTS. HOWEVER, DIRECT ENZYME REPLACEMENT HAS LED TO HYPERSENSITIVITY RESPONSES IN THE HOSTS, AND INSTABILITY OF SUCH PREPARATIONSHAS MADE USE OF SUCH PROCEDURES IMPRACTICAL. WE PROPOSE TO EXPLORE IMMOBILIZATION CHEMISTRIES FOR DEVELOPMENT OF ENZYME-ACTIVATED SURFACES FOR EXTRACORPOREAL REMOVAL OF TOXIC METABOLITIES. WE WILL USE PHOTOCHEMICAL PROCEDURES TOACTIVATE SUPPORT MATRICES FOR SUBSEQUENT COUPLING TO THE MODEL ENZYME, ARGINASE. SUCCESSFUL DEMONSTRATION OF THE IMPROVED ENZYME ACTIVITIES AFFORDED BY THIS TECHNOLOGY IN ANIN VITRO BLOOD SYSTEM WILL LEAD TO CONTINUATION OF THIS PROJECT WITH A CONSULTANT LABORATORY FOR PHASE II ANIMAL MODEL STUDIES.

* Information listed above is at the time of submission. *

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