AFFINITY COUPLING OF RECEPTORS ON MICROCHIPS

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$50,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
10789
Agency Tracking Number:
10789
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9924 W 74th St, Eden Prairie, MN, 55337
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Patrick E Guire
Principal Investigator
(612) 829-2733
Business Contact:
() -
Research Institute:
n/a
Abstract
REAGENTS AND REACTION PROCEDURES WILL BE DEVELOPED DURING PHASE I FOR THE COUPLING OF ANTIBODY MOLECULES TO THE ACTIVATED SURFACE OF MICROCHIP CAPACITANCE SENSORS. PROPRIETARY PHOTOAFFINITY REAGENTS WILL BE SYNTHESIZED AND COUPLED TO THE ELECTRODE SURFACE FOR SPECIFIC COVALENT BINDING OF MONOCLONAL ANTIBODY (USING ONE OF THE TWO ANTIBODY BINDING SITES). THIS WILL LEAVE THE REMAINING STABILIZED ANTIBODY BINDING SITE AVAILABLE FOR FUNCTIONAL EXCHANGE BETWEEN SURFACE-IMMOBILIZED ANALYTE (TOLUENE OR DERIVATIVES) AND FREE ANALYTE MOLECULES, IF PRESENT, IN THE SAMPLE. PRESENCE OF ANALYTE IN THE SAMPLE WILL INDUCE DISPLACEMENT OF ANTIBODY FROM SURFACE-IMMOBILIZED ANALYTE. THE CAPACITANCE WILL INCREASE PROPORTIONALLY TO THE AMOUNT OF FREE ANALYTE IN THE SAMPLE. A CURRENT VERSION OF THIS IMMUNOSENSOR HAS THE DETECTING REAGENTS ENVELOPED IN A SEMIPERMEABLE MEMBRANE. ACHIEVEMENT OF THE OBJECTIVES OF THIS PROJECT WILL ELIMINATE THE NEED FOR THIS MEMBRANE AND WILL DEMONSTRATE A SENSOR WITH IMPROVED PERFORMANCE (FASTER RESPONSE AND REGENERATION AS WELL AS INCREASED SENSITIVITY, PARTICULARLY WHEN RAPID ANALYSES ARE REQUIRED). DEMONSTRATING SENSOR FEASIBILITY WITH TOLUENE (OR DERIVATIVES) WILL SUPPORT THE UTILITY OF THIS TECHNOLOGY FORSENSITIVE , RAPID, AND SELECTIVE SENSING AND MONITORING OF VARIOUS ADVERSE AGENTS. THE SENSOR DEVICE WILL REVERSIBLY RECOGNIZE AND QUANTITATE ANALYTE CONTENT IN BATCH OR CONTINUOUS SAMPLING SITUATIONS AND TRANSDUCE THE SIGNAL WITHELECTRONIC DATA RECORDING AND TRANSMISSION. SUCH A DEVICE WOULD OFFER A BROAD RANGE OF APPLICABILITY FOR PERIODIC SAMPLE ANALYSIS AND REAL-TIME, ON-LINE MONITORING OF FLUIDS (AIR, WATER, AND FUELS) AS WELL AS DOSIMETRY MEASUREMENTS INAIR AND PHYSIOLOGICAL FLUIDS.

* information listed above is at the time of submission.

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