IMMOBILIZATION OF BIOMOLECULES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$363,744.00
Award Year:
1990
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
14228
Agency Tracking Number:
14228
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9924 W 74th St, Eden Prairie, MN, 55344
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Patrick E Guire
(612) 829-2700
Business Contact:
() -
Research Institution:
n/a
Abstract
IMMOBILIZATION OF BIOMOLECULES RESULTS IN GREAT LOSSES OF SPECIFIC ACTIVITIES OF THE IMMOBILIZED MOIETIES. THE USE OF HYDROPHILIC SPACERS BETWEEN THE BIOMOLECULES AND THE MATRIX RESULTS IN IMPROVED ACTIVITIES. HOWEVER, FACILE INTRODUCTION OF SUCH SPACERS ONTO MANY INSOLUBLE SUPPORTS ISDIFFICULT WITHOUT CAUSING DAMAGE TO THE SUPPORT OR THE USE OF SOPHISTICATED CHEMISTRY. DURING PHASE I, IT WAS DEMONSTRATED THAT SEVERAL MATRICES COULD BE DERIVATIZED WITH HYDROPHILIC SPACERS. COUPLING OF MODEL MONOCLONAL ANTIBODIES AND THEIR COMPLEMENTARY ANTIGENSTO THESE SPACER-DERIVATIZED SURFACES LED TO GREATER TOTAL AND SPECIFIC IMMUNOACTIVITIES AS COMPARED TO DIRECT COUPLINGPROCEDURES. PHASE II EFFORTS WILL OPTIMIZE THE CHEMICAL PROCEDURES TO DEVELOP MATRICES DERIVATIZED WITH HYDROPHILIC SPACERS, WHICHWILL BE OF BENEFIT TO ALL USERS OF MICROTITER PLATES, POLYSTYRENE BEADS, GEL MEDIA, OR OTHER MATRICES TO WHICH BIOMOLECULES ARE COMMONLY COUPLED. THE TECHNOLOGY THAT IS EXPECTED TO RESULT FROM THIS PROJECT WILL LEAD TO THE DEVELOPMENT OF SEVERAL SUPPORT MATERIALS THAT WILL BE OPTIMIZED FOR FACILE, SPECIFIC COUPLING OF BIOMOLECULES, SUCH AS ANTIBODIES, ANTIGENS, AND ENZYMES, ANDWILL GREATLY INCREASE THE TOTAL AND SPECIFIC ACTIVITIES OF THE IMMOBILIZED MOLECULES. AWARDEE'S STATEMENT OF THE POTENTIAL COMMERCIAL APPLICATIONSOF THE RESEARCH: COVALENT BINDING OF BIOMOLECULES AND THE USE OF OPTIMIZED SPACERS ON MICROTITER PLATES, GEL MEDIA, AND POLYSTYRENE BEADS WILL PROVIDE SUPPORTS USEFUL FOR THE DEVELOPMENT OF NEW AND/OR IMPROVED ASSAYS FOR SPECIFIC ANALYTE DETECTION AND SUPPORTS FOR PURIFICATION OF BIOMOLECULES. INCREASED LOADING OF BIOMOLECULES WIHT INCREASED BIOMOLECULE ACTIVITIES WILL MAKE THESE SUPPORTS ATTRACTIVE FOR MANY APPLICATIONS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government