RECEPTOR IMMOBILIZATION FOR AFFINITY SEPARATIONS

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$245,718.00
Award Year:
1994
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
14278
Agency Tracking Number:
14278
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9924 West Seventy-fourth St, Eden Prairie, MN, 55344
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Melvin J. Swanson, Ph.d.
Principal Biochemist
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
A PROJECT IS PROPOSED TO DEVELOP REAGENTS AND METHODS FOR IMMOBILIZING RECEPTORS ONTO POLYSULFONE MICROPOROUS MEMBRANES USED FOR AFFINITY PURIFICATION, BY PHOTOIMMOBILIZING HYDROPHILIC POLYMERIC COUPLING REAGENTS TOWHICH THE RECEPTOR WILL THEN BE COUPLED. THE TECHNIQUE IS EXPECTED TO INCREASE THE AMOUNT OF ACTIVE RECEPTOR IMMOBILIZED, TO REDUCE NONSPECIFIC BINDING AND TO INCREASE STABILITY OF THE IMMOBILIZED RECEPTOR OR ANTIBODY MOLECULES. THE TECHNOLOGY WILL BE DEVELOPED USING A MODEL ANTIBODY AFTER WHICH IT WILL ALSO BE EVALUATED FOR EFFICIENCY OF IMMOBILIZING STAPHYLOCOCCUS AUREUS PROTEIN A. USE OF THESE METHODS FOR PREPARING AFFINITY MEMBRANES IS EXPECTED TO INCREASE THE EFFICIENCY OF THE PURIFICATION PROCESS AND TO REDUCE THE COSTS OF A VARIETY OF BIOSEPARATIONS, INCLUDING PURIFICATION OF RECOMBINANT PROTEINS, ANTIBODIES AND PLASMA PROTEINS FROM CELL CULTURE SUPERNATANTS, PLASMA AND ASCITES FLUID.

* information listed above is at the time of submission.

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