DEVELOPMENT OF A DNA PROBE FOR THE RAPID DETECTION OF OF MYCOMPLASMA GALLISEPTICUM

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$150,198.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
1900
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Bionique Laboratorytories I
Bloomingdale Road, Saranac Lake, NY, 12983
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
STEVEN J. GEARY
PRINCIPAL INVESTIGATOR
(518) 891-2356
Research Institution:
n/a
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO CONSTRUCT A MYCOMPLASMA GALLISEPTICUM BIOTINYLATED DNA PROBE. ECONOMIC LOSSES FROM M. GALLISEPTICUM INFECTION ARE ESTIMATED TO BE $118 MILLION ANNUALLY IN THE UNITED STATES. MINIMUM EFFICIENCY OF M. GALLISEPTICUM DISEASE CONTROL REQUIRES A RAPID AND SENSITIVEIDENTIFICATION SYSTEM SUCH AS A PROBE. MYCOPLASMA GAL GALLISEPTICUM DNA WILL BE LABELED WILL BIOTIN-11-DUTP IN A STANDARD NICK-TRANSLATION REACTION. TARGET DNA HYBRIDIZED WITH THE BIOTINYLATED PROBE ON A NITROCELLULOSE FILTER WILL BE INCUBATED WITH STREPTAVIDIN AND POLYALKALINE PHOSPHASTASE. UPON ADDITION OF AN ENZYME/SUBSTRATE COMPLEX, THE HYBRIDIZED DNA WILL APPEAR AS A BLUE SPOT. RESULTS CAN BE OBTAINED IN 24-36 HOURS, WHICH IS CONSIDERABLY FASTER THAN ROUTINE METHODS CURRENTLY IN USE.

* information listed above is at the time of submission.

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