DEVELOPMENT OF A DNA PROBE FOR THE RAPID DETECTION OF OF MYCOMPLASMA GALLISEPTICUM

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: N/A
Agency Tracking Number: 1900
Amount: $150,198.00
Phase: Phase II
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Bionique Laboratorytories I
Bloomingdale Road, Saranac Lake, NY, 12983
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
 STEVEN J. GEARY
Title: PRINCIPAL INVESTIGATOR
Phone: (518) 891-2356
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO CONSTRUCT A MYCOMPLASMA GALLISEPTICUM BIOTINYLATED DNA PROBE. ECONOMIC LOSSES FROM M. GALLISEPTICUM INFECTION ARE ESTIMATED TO BE $118 MILLION ANNUALLY IN THE UNITED STATES. MINIMUM EFFICIENCY OF M. GALLISEPTICUM DISEASE CONTROL REQUIRES A RAPID AND SENSITIVEIDENTIFICATION SYSTEM SUCH AS A PROBE. MYCOPLASMA GAL GALLISEPTICUM DNA WILL BE LABELED WILL BIOTIN-11-DUTP IN A STANDARD NICK-TRANSLATION REACTION. TARGET DNA HYBRIDIZED WITH THE BIOTINYLATED PROBE ON A NITROCELLULOSE FILTER WILL BE INCUBATED WITH STREPTAVIDIN AND POLYALKALINE PHOSPHASTASE. UPON ADDITION OF AN ENZYME/SUBSTRATE COMPLEX, THE HYBRIDIZED DNA WILL APPEAR AS A BLUE SPOT. RESULTS CAN BE OBTAINED IN 24-36 HOURS, WHICH IS CONSIDERABLY FASTER THAN ROUTINE METHODS CURRENTLY IN USE.

* information listed above is at the time of submission.

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