DEVELOPMENT OF A DNA PROBE FOR THE RAPID DETECTION OF OF MYCOMPLASMA GALLISEPTICUM

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$150,198.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
1900
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Bionique Laboratorytories I
Bloomingdale Road, Saranac Lake, NY, 12983
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
STEVEN J. GEARY
PRINCIPAL INVESTIGATOR
(518) 891-2356
Research Institution:
n/a
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO CONSTRUCT A MYCOMPLASMA GALLISEPTICUM BIOTINYLATED DNA PROBE. ECONOMIC LOSSES FROM M. GALLISEPTICUM INFECTION ARE ESTIMATED TO BE $118 MILLION ANNUALLY IN THE UNITED STATES. MINIMUM EFFICIENCY OF M. GALLISEPTICUM DISEASE CONTROL REQUIRES A RAPID AND SENSITIVEIDENTIFICATION SYSTEM SUCH AS A PROBE. MYCOPLASMA GAL GALLISEPTICUM DNA WILL BE LABELED WILL BIOTIN-11-DUTP IN A STANDARD NICK-TRANSLATION REACTION. TARGET DNA HYBRIDIZED WITH THE BIOTINYLATED PROBE ON A NITROCELLULOSE FILTER WILL BE INCUBATED WITH STREPTAVIDIN AND POLYALKALINE PHOSPHASTASE. UPON ADDITION OF AN ENZYME/SUBSTRATE COMPLEX, THE HYBRIDIZED DNA WILL APPEAR AS A BLUE SPOT. RESULTS CAN BE OBTAINED IN 24-36 HOURS, WHICH IS CONSIDERABLY FASTER THAN ROUTINE METHODS CURRENTLY IN USE.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government