Large Throughput Multiplex Analysis of Gene Expression

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 CA68853-01,
Agency Tracking Number:
29250
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Bios Laboratorytories, Inc.
5 Science Park, New Haven, CT, 06511
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Gualberto Ruano
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
We will develop resources for large-throughput analysis of gene expression. The complement of genesexpressed in a given cell varies during development, in disease states and in response to hormonal sgene expression are the basis of cancer. This project will show the feasibility of "Multiplex Profillarge-throughput strategy for characterizing changes in gene expression and for retrieving the variaperform MP on gene transcripts synthesized by two methods targeted to open reading frames and 5' regdevelop conditions for multiplex electrophoresis of PCR products and for hybridization and retrievaltranscripts with "signature" oligonucleotides derived from conserved protein motifs. MP will be appland normal respiratory epithelium cell lines using signature oligos for G proteins. The differentialcould become molecular markers for these tumors.

* information listed above is at the time of submission.

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