Novel Repeat Elements for Genome Analysis
Department of Health and Human Services
1 R43 CA71315-01,
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Small Business Information
Bios Laboratorytories, Inc.
5 Science Park, New Haven, CT, 06511
Socially and Economically Disadvantaged:
AbstractThe goal of this Phase I project is to isolate and characterize new chromosome-specific human repetelements. The resulting probes will be valuable for cytogenetic cancer diagnostics. Our preliminaryrandom amplification of polymorphic DNA (RAPD) is a valuable approach for the discovery of chromosompolymorphic repetitive DNA elements. Phase I will involve systematic and high- throughput employmentgenome-wide search for such elements. The repetitive DNAs will be cloned, then characterized by sequchromosomal localization and sublocalization, heterozygosity determination, evaluation of evolutionadetermination of average copy number. Additionally, Phase I will include modeling cytogenetic analyschromosomal aberrations using chromosome-specific repetitive DNA probes. Our model system will emplorepetitive element mapping to chromosome 20q12, which we discovered in preliminary research using RAprobe in interphase nuclei representing specific hematological malignancies known to have chromosomavicinity
* information listed above is at the time of submission.