Directed Multiplex DNA Sequencing by Hybridization

Award Information
Agency: Department of Energy
Branch: N/A
Contract: DE-FG02-96ER82136
Agency Tracking Number: 34690
Amount: $750,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1997
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Genaissance Pharmaceuticals
Five Science Park, New Haven, CT, 06511
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Dr. Gualberto Ruano
 Scientific Director
 (203) 773-1450
Business Contact
 Mr. Kevin Rakin
Title: Chief Financial Officer
Phone: (203) 773-1450
Research Institution
N/A
Abstract
41186 BIOS Laboratories, Inc. The ultimate goal of the Human Genome program is to obtain the entire sequence of the genetic makeup of the human body. This research has mostly encompassed large scale DNA sequencing projects, with interdisciplinary teams of biochemists, engineers, and computer specialists, which are beyond the resources of small to mid-sized laboratories. This project will develop an alternate, lower cost technique for directed DNA sequencing, utilizing hybridizations of Peptide Nucleic Acid (PNA) oligomer probes. PNA oligomers hybridize more stably and specifically to DNA targets than conventional oligonucleotides. Phase I is concerned with improvements in the three multiply repeated steps in the directed sequencing process: (1) probe assembly, (2) sequencing reactions and (3) gel electrophoresis. The technique allows the efficiency of directed sequencing, while avoiding most of the bottlenecks identified for the process. Commercial Applications and Other Benefits as described by the awardee: The development of modular PNA probes from pre-synthesized libraries should substantially reduce the cost of PNAs and extend further the applications of these novel molecules to DNA sequencing. The resources to be developed include PNA oligomer libraries, anchor primer kits for multiplex sequencing, and customized services.

* information listed above is at the time of submission.

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