USE OF DNA PROBES FOR THE DETECTION OF LYME DISEASE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13606
Amount: $49,110.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Biotech Research Laboratories
1600 E Gude Dr, Rockville, MD, 20850
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Mark M Manak
 (301) 251-0800
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE TREATMENT OF LYME DISEASE COULD BE GREATLY ENHANCED IF IT WERE POSSIBLE TO DETECT THE LYME DISEASE SPIROCHETE DIRECTLY IN BLOOD WITH A HIGHLY SENSITIVE TEST. IT IS PLANNED TO DEVELOP A TEST WHERE A REGION OF THE BORRELIA BURGDORFERI OSPA GENE IS AMPLIFIED USING THE POLYMERASE CHAIN REACTION (PCR) TO OBTAIN THE NECESSARY SENSITIVITY. THE AMPLIFIED PRODUCT WILL BE DETECTED INITIALLY BY SOUTHERNBLOTTING AND EVENTUALLY BY SANDWICH HYBRIDIZATION IN MICROTITER WELLS USING A NONRADIOACTIVE DNA PROBE. IN PHASE I, THE REAGENTS NECESSARY FOR THE PCR AMPLIFICATION OF B. BURGDORFERI DNA WILL BE DEVELOPED, AND, IF POSSIBLE, THE FEASIBILITY OF DETECTING THE SPIROCHETE USING THE PCR AND SOUTHERN BLOTTING WILL BE TESTED. A DIRECT AND SENSITIVE TEST OF THIS TYPE FOR LYME DISEASE SHOULD PERMIT EARLIER DETECTION, BETTER DIAGNOSTIC CONFIRMATION, AND EFFECTIVE MONITORING OF ANTIBIOTIC THERAPY.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government