TISSUE HOMOGENIZATON BY PRESSURE CYCLING TECHNOLOGY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$0.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
2R44RR014988-02
Award Id:
60106
Agency Tracking Number:
RR014988
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
BIOSEQ, INC., 217 PERRY PKY, GAITHERSBURG, MD, 20877
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
FENG TAO
(301) 208-8100
FTAO@BBII.COM
Business Contact:
MARK MANAK
(301) 208-8100
MMANAK@BBII.COM
Research Institution:
n/a
Abstract
An application using pressure cycling technology (PCT) is being developed to lyse cells and homogenize tissues for automated biological sample processing. This technology is feasible for automated processes in both low and high throughput applications. Our Phase I study demonstrated that PCT is very powerful to homogenize both animal and plant tissue samples. Nucleic acids released by PCT were comparable to conventional methods, and suitable for PCR and RT-PCR analysis. Bacteria samples could be lysed in simple lysis solution and crude lysate generated by PCT was suitable for PCR amplification. In this phase II study, tissue homogenization will be evaluated with a variety of animal and plant samples with different degrees of difficulties. Compatibility of the PCT tissue lysis procedure and the general nucleic acid extraction methods will be evaluated. Multiple parallel sample processing will be developed. The final objective of the project is to demonstrate utilities of PCT lysate in a variety of biological assays using nucleic acids and proteins. Steps towards commercialization of this homogenization process will be pursued upon completion of this Phase II study.

* information listed above is at the time of submission.

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