Multiplex RT-PCR Test for HTLV-I/II, HIV-1, HBV and HCV

A novel, unique, rapid and reliable multiplex RT-PCR test will be developed for the simultaneous deviral pathogens, HTLV- I/II, HIV-1, HBV and HCV, frequently associated with transfusion- transmittedtranscription of HCV RNA, and amplification of HTLV-I/ II, HIV-1 and HBV DNAs will be carried out sisingle reaction tube using a combination of primers specific for these pathogens. The PCR products wmicrotiter capture plates designed for their simultaneous or individual detection. Simplified procedestablish the optimum conditions for rapid collection and processing of whole blood samples, while pof both the RNA and DNA. The transcription and amplification conditions for the simultaneous amplifianalytes in a single reaction tube using a multiplex RT-PCR approach will be worked out. The sensitireproducibility of multiplex RT-PCR and detection assay will be validated on appropriate quality consamples from infected individuals. In the course of Phase II, these studies will be extended to furtmultiplex RT-PCR kits for suitability of use in a clinical laboratory setting.