Multiplex RT-PCR Test for HTLV-I/II, HIV-1, HBV and HCV

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 HL54370-01,
Agency Tracking Number: 29534
Amount: $750,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1997
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
3 Taft Court, Rockville, MD, 20850
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Lucia Gonzalez-Villasenor
 () -
Business Contact
Phone: () -
Research Institution
A novel, unique, rapid and reliable multiplex RT-PCR test will be developed for the simultaneous deviral pathogens, HTLV- I/II, HIV-1, HBV and HCV, frequently associated with transfusion- transmittedtranscription of HCV RNA, and amplification of HTLV-I/ II, HIV-1 and HBV DNAs will be carried out sisingle reaction tube using a combination of primers specific for these pathogens. The PCR products wmicrotiter capture plates designed for their simultaneous or individual detection. Simplified procedestablish the optimum conditions for rapid collection and processing of whole blood samples, while pof both the RNA and DNA. The transcription and amplification conditions for the simultaneous amplifianalytes in a single reaction tube using a multiplex RT-PCR approach will be worked out. The sensitireproducibility of multiplex RT-PCR and detection assay will be validated on appropriate quality consamples from infected individuals. In the course of Phase II, these studies will be extended to furtmultiplex RT-PCR kits for suitability of use in a clinical laboratory setting.

* Information listed above is at the time of submission. *

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