Selective Silver Staining of DNA Probes

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 GM52768-01,
Agency Tracking Number: 29454
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1995
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
620 Hutton Street, Suite 104, Raleigh, NC, 27606
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Bernard Spielvogel
 () -
Business Contact
Phone: () -
Research Institution
The major impediment to the wide implementation of DNA diagnostic methods is their cost. Technologisimplify current DNA diagnostic methods and to make them cost competitive with antibody based assaysdesirable. Our new selective silver staining technology could dramatically enhance the price/performand the speed of many currently available DNA based diagnostic methods. Compared to leading alternatpotential advantages of our proprietary technology are reduced material costs, faster detection proc(eliminating radioisotopes or toxic dyes), greater ease of use for manual systems and greater ease oresults with model compounds clearly show the possibility of selectively staining DNA probes. Phasedetermining feasibility with large probe size DNA fragments in real situations on gels or membranes,limit of detection. Methods will also be investigated to improve the yield of modified nucleotides rof probes. Phase II studies will focus on methods of quantitation, demonstration of feasibly with cldiagnostic methods using selective silver staining.

* Information listed above is at the time of submission. *

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