RECENTLY, SUBSTANCES WITH INTRINSIC NATRIURETIC, DIURETIC AND VASORELAXANT ACTIVITY HAVE BEEN ISOLATED FROM THE ATRIUM OF THE HEART.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
3221
Agency Tracking Number:
3221
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2450 Bayshore Frontage Rd., Mountain View, CA, 94043
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN A. LEWICKI
PRINCIPAL INVESTIGATOR
(415) 966-1550
Business Contact:
() -
Research Institution:
n/a
Abstract
RECENTLY, SUBSTANCES WITH INTRINSIC NATRIURETIC, DIURETIC AND VASORELAXANT ACTIVITY HAVE BEEN ISOLATED FROM THE ATRIUM OF THE HEART. STUDIES OF THESE SUBSTANCES, TERMED ATRIAL NATRIURETIC FACTORS (ANF), HAVE PROVIDED NEW INFORMATION RELEVANT TO OUR UNDERSTANDING OF THE REGULATION OF BLOOD PRESSURE AND FLUID VOLUME. IN ADDITION, IT IS PROPOSED THAT STUDIES OF THESE AGENTS WILL LEAD TO NOVEL THERAPEUTIC APPROACHES FOR TREATING HYPERTENSION AND VARIOUS EDEMATOUS STATES. ALTHOUGH BIOLOGICALLY ACTIVE ANF PEPTIDES OF 2128 AMINO ACIDS HAVE BEEN ISOLATED AND SEQUENCED, IT IS NOW APPARENT THAT THESE PEPTIDES RESIDE IN ATRIUM PREDOMINANTLY AS A 126 AMINO ACID PRECURSOR FORM (PRO ANF). DUE TO THE LIMITED QUANTITIES AVAILABLE FROM NATURAL SOURCES, NOTHING IS KNOWN ABOUT THE PHYSIOLOGICAL ACTIONS OR METABOLIC FATE OF PRO ANF. HAVING ISOLATED A CDNA CLONE ENCODING RATE PRE-PRO ANF, WE PROPOSE TO EXPRESS PRO ANF IN E. COLI. FOLLOWING PURIFICATION, RECOMBINANT PRO ANF WILL BE TESTED FOR BIOLOGICAL ACTIVITY IN MODEL TEST SYSTEMS. IN ADDITION, THE CIRCULATORY FATE OF PRO ANF WILL BE EXAMINED. THE PROPOSED STUDIES WILL PROVIDE RELEVANT INFORMATION ABOUT THE GENERAL ROLE AND FUNCTION OF PRO ANF AND WILL TEST THE CONCEPT THAT PRO ANF HAS INHERENT THERAPEUTIC VALUE FOR TREATING CARDIOVASCULAR DISEASE.

* information listed above is at the time of submission.

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