ANGIOGENESIS WITH ANTAGONIST AND AGONIST ANALOGS OF BASIC FGF

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$494,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
7314
Agency Tracking Number:
7314
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2450 Bayshore Parkway, Mountain View, CA, 94043
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Andrew A Protter Phd
(415) 966-1550
Business Contact:
() -
Research Institute:
n/a
Abstract
BASIC FIBROBLAST GROWTH FACTOR (BFGF) IS A POTENT MITOGEN AND ANGIOGENESIS-PROMOTING FACTOR. ANALOGS OF BFGF WILL BE SYNTHESIZED BY IN VITRO MUTAGENESIS OF A DNA FRAGMENT CODINGFOR HUMAN BFGF. THE MUTATED BFGF-CODING DNA FRAGMENT WILL BE ENGINEERED INTO A BACTERIAL EXPRESSION VECTOR AND TRANSFECTED INTO BACTERIA. THE BFGF ANALOGS PRODUCED BY THEBACTERIA WILL BE PURIFIED BY HEPARIN-SEPHAROSE CHROMATOGRAPHY AND TESTED FOR AGONIST AND ANTAGONIST ACTIVITY TO BOVINE PITUITARY BFGF USING TWO DIFFERENT CELL ASSAYS AND A BFGF-RECEPTOR-BINDING ASSAY. THIS IS A FIRST STEP TOWARD DEVELOPING POTENTIAL THERAPEUTICS FOR REGULATINGANGIOGENESIS BASED ON THE STRUCTURE OF BFGF.

* information listed above is at the time of submission.

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