EXPRESSION VECTORS FOR SECRETED HUMAN ESTROGEN RECEPTOR

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
7189
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
California Biotechnology Inc
2450 Bayshore Frontage Road, Mountain View, CA, 94043
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PHYLLIS A PONTE PHD
(415) 966-1550
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG-TERM GOAL OF THE RESEARCH IS TO OBTAIN A CHINESE HAMSTER OVARY CELL LINE CAPABLE OF PRODUCING HUMAN ESTROGEN RECEPTOR AS A SIGNIFICANT AMOUNT OF ITS SECRETED PROTEIN. PURIFIED RECEPTOR PROTEIN MAY THEN BE USED (1) TO OBTAIN X-RAY CRYSTALLOGRAPHIC DATA ON THE STRUCTURE OF THE RECEPTOR TO AID IN RATIONAL ANTI-ESTROGEN DRUG DESIGN, AND (2) TO ACT AS A RESEARCH REAGENT FOR THE DEVELOPMENT OF ESTROGEN ANTAGONISTS FOR TREATMENT OF ESTROGEN RECEPTOR TUMORS. THE SPECIFIC AIM OF THE PROPOSAL IS THE PRODUCTION OF A MAMMALIAN CELL EXPRESSION VECTOR CAPABLE OF ENCODING A SECRETED ESTROGEN RECEPTOR AND THE PRODUCTION OF CHO CELL TRANSFECTANT POOLS USING THIS VECTOR. A PRIMARY ACCOMPLISHMENT WILL BE THE INVENTION OF EXPRESSION VECTORS ALLOWING FOR THE RAPID CONSTRUCTION AND IN VITRO ANALYSIS OF ANY NOVEL SECRETORY PROTEIN.

* information listed above is at the time of submission.

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