EXPRESSION VECTORS FOR SECRETED HUMAN ESTROGEN RECEPTOR

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7189
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1987
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
California Biotechnology Inc
2450 Bayshore Frontage Road, Mountain View, CA, 94043
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 PHYLLIS A PONTE PHD
 (415) 966-1550
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM GOAL OF THE RESEARCH IS TO OBTAIN A CHINESE HAMSTER OVARY CELL LINE CAPABLE OF PRODUCING HUMAN ESTROGEN RECEPTOR AS A SIGNIFICANT AMOUNT OF ITS SECRETED PROTEIN. PURIFIED RECEPTOR PROTEIN MAY THEN BE USED (1) TO OBTAIN X-RAY CRYSTALLOGRAPHIC DATA ON THE STRUCTURE OF THE RECEPTOR TO AID IN RATIONAL ANTI-ESTROGEN DRUG DESIGN, AND (2) TO ACT AS A RESEARCH REAGENT FOR THE DEVELOPMENT OF ESTROGEN ANTAGONISTS FOR TREATMENT OF ESTROGEN RECEPTOR TUMORS. THE SPECIFIC AIM OF THE PROPOSAL IS THE PRODUCTION OF A MAMMALIAN CELL EXPRESSION VECTOR CAPABLE OF ENCODING A SECRETED ESTROGEN RECEPTOR AND THE PRODUCTION OF CHO CELL TRANSFECTANT POOLS USING THIS VECTOR. A PRIMARY ACCOMPLISHMENT WILL BE THE INVENTION OF EXPRESSION VECTORS ALLOWING FOR THE RAPID CONSTRUCTION AND IN VITRO ANALYSIS OF ANY NOVEL SECRETORY PROTEIN.

* information listed above is at the time of submission.

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