ISOLATION OF INSULIN RECEPTOR PHOSPHATASES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
16535
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
California Biotechnology, Inc
2450 Bayshore Parkway, Mountain View, CA, 94043
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Sweet, Laurel J
Principal Investigator
(415) 966-1550
Business Contact:
1 R43 DK44046-01
() -
Research Institution:
n/a
Abstract
THE ULTIMATE OBJECTIVE OF THIS RESEARCH IS TO DEVELOP A NOVEL TREATMENT FOR DIABETES BASED ON THE ENHANCEMENT OF INSULIN ACTION THROUGH THE INHIBITION OF AN INSULIN RECEPTOR-SPECIFIC PHOSPHOTYROSINE PHOSPHATASE (PTPASE). PHOSPHORYLATION OF THE INSULIN RECEPTOR BETA-SUBUNIT, IN RESPONSE TO INSULI BINDING, HAS BEEN CORRELATED WITH ACTIVATION OF THE PLEIOTROPHIC INTRA- CELLULAR INSULIN SIGNAL. MODULATION OF THE PHOSPHORYLATION STATE OF THE INSULIN RECEPTOR COULD AFFECT POST-RECEPTOR SIGNALLING EVENTS. THE SPECIFIC AIMS OF THIS PROJECT ARE TO IDENTIFY AND PURIFY AS INSULIN RECEPTOR PTPASE(S). DURING PHASE I, WE WILL IDENTIFY AND PARTIALLY PURIFY AN INSULIN RECEPTOR-SPECIFIC PTPASE. THE SPECIFICITY OF THE PHOSPHATASE WILL BE EXAMINED COMPARING DEPHOSPHORYLATION OF THE INSULIN RECEPTOR TO OTHER PHOSPHOTYROSINE, PHOSPHOSERINE, AND PHOSPHOTHREONINE-CONTAINING SUBSTRATES. DURING PHASE II, WE WILL CONTINUE PURIFICATION, GENERATE MONOCLONAL ANTIBODIES, AND IDENTIFY THE CDNA ENCODING THE INSULIN RECEPTOR PTPASE ACTIVITY.

* information listed above is at the time of submission.

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