THE BIODEGRADATION OF CHLORINATED ALIPHATIC ALIPHATIC COMPOUNDS BY METHANE-UTILIZING BACTERIA: MECHANISM AND PRODUCTS

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 2613
Amount: $200,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Cambridge Analytical Assoc Inc
1106 Commonwealth Ave, Boston, MA, 02215
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Samuel Fogel
 Dir Chemical Cons Div
 (617) 232-2207
Business Contact
Phone: () -
Research Institution
N/A
Abstract
LOW MOLECULAR WEIGHT CHLORINATED HYDROCARBONS ARE WIDESPREADENVIRONMENTAL CONTAMINANTS AND ARE OF CONCERN BECAUSE OF THEIR TOXIC AND CARCINOGENIC PROPERTIES. THEY ARE PERSISTENT IN SOILS AND GROUNDWATER, RESIST DEGRADATION IN BIOLOGICAL WASTEWATER TREATMENT SYSTEMS, AND ARE EXPENSIVE TO REMOVE FROM WATER BY PHYSICAL/CHEMICAL PROCESSES. WE HAVE ISOLATED A BACTERIUM WHICH GROWS BY OXIDIZING METHANE (A METHANOTROPH) AND WHICH CAN ALSO OXIDIZE AND DEGRADE VINYL CHLORIDE, VINYLIDENE CHLORIDE, ALL DICHLOROETHYLENES, AND TRICHLOROETHYLENE, PLUS TWO CHLORINATED ETHANES AND TWO CHLOROMETHANES. THIS DISCOVERY HAS SIGNIFICANT POTENTIAL FOR APPLICATIONS IN THE FIELD OF GROUNDWATER AND WASTEWATER TREATMENT. OUR PRELIMINARY EXPERIMENTS WITH 14C-TRICHLOROETHYLENE BIODEGRATDATION DEMONSTRATED THAT AT LEAST 60% OF THE 14C COULD BE RECOVERED AS 14C02 AND 14C-BIOMASS. BASED ON REPORTED REACTION MECHANISMS FOR THE OXIDATION OF CHLORINATED ETHENES IN MAMMALIAN SYSTEMS, WE HAVE DETERMINEDTHAT THE INITIAL OXIDATION PRODUCTS SHOULD BE DEGRADABLE BY BACTERIA. WE PROPOSE TO CARRY OUT EXTENSIVE CHEMICAL ANALYSIS OF THE PRODUCTS OF METHANOTROPHIC DEGRADATION OF THE SELECTED CONTAMINANTS IN ORDER TO DEMONSTRATE FOR EACH ONE THAT NO TOXIC PRODUCTS ARE FORMED. WE ALSO PROPOSE TO OPERATE A LABORATORY-SCALE TREATMENT COLUMN EMPLOYING OUR METHANOTROPHIC ISOLATE AND TO OPTIMIZE CONDITIONS FOR THE REMOVAL OF CHLORINATED ALIPHATICS FROM CONTAMINATED WATER.

* information listed above is at the time of submission.

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