A NOVEL CYTOCHEMICAL BIOASSAY FOR PARATHYROID HORMONE

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
7220
Agency Tracking Number:
7220
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
575 Middlesex Turnpike, Billerica, MA, 01865
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
CAROL VARANELLI
(617) 667-2161
Business Contact:
() -
Research Institute:
n/a
Abstract
RADIOIMMUNOASSAYS (RIAS) REPRESENT THE STANDARD METHODOLOGY FOR THE MEASUREMENT OF PARATHYROID HORMONE (PTH). HOWEVER, THERE ARE A NUMBER OF LIMITATIONS ASSOCIATED WITH THE MEASUREMENT OF PTH BY THIS TECHNIQUE. MOST IMMUNOASSYS ARE INSENSITIVE AND, THEREFORE, CANNOT DETECT CIRCULATING LEVELSOF HORMONE IN NORMAL SUBJECTS. CONSEQUENTLY, RIAS CANNOT DISTINGUISH NORMAL FROM HYPOPARATHYROID LEVELS OF PTH. MOSTRIAS ARE HETEROLOGOUS SYSTEMS THAT OFTEN UTILIZE BOVINE PTH AS THE RADIOLIGAND AND ANTISERA RAISED AGAINST BOVINE PTH TO MEASURE HUMAN PTH. A MAJOR FACTOR COMPLICATING PTH-RIA TECHNIQUES IS THE PRESENCE OF MULTIPLE FORMS OF CIRCULATING HORMONE. A PREPONDERANCE OF MIDREGION/CARBOXY-TERMINAL HORMONE FRAGMENTS EXIST IN THE CIRCULATION, BUT THESE FORMS OF THE PEPTIDE ARE WITHOUT BIOLOGICAL ACTIVITY AND HAVE PROLONGED HALF-LIVES COMPARED TO BIOLOGICALLY ACTIVE INTACT PTH (1-84). THE HETEROGENEITY OF THE HORMONE CONTRIBUTES TO THEHIGH NOISE-TO-SIGNAL RATIO ENCOUNTERED IN MOST PTH-RIAS. THE CYTOCHEMICAL BIOASSAY (CBA) FOR PTH CIRCUMVENTS THE PROBLEMS ASSOCIATED WITH RIA AND REPRESENTS THE MOST SENSITIVE METHOD CURRENTLY AVAILABLE FOR MEASURING THIS HORMONE, BUT THIS ASSAY HAS NOT GAINED WIDESPREAD USE BECAUSE IT IS LABOR INTENSIVE, HAS LIMITED SAMPLE THROUGHPUT, AND REQUIRES EXPENSIVE INSTRUMENTATION. PRELIMINARY DATA SUGGEST THAT THIS SENSITIVE ASSAY SYSTEM CAN BE MODIFIED, CREATING THE OPPORTUNITY TO DEVELOP A POWERFUL DIAGNOSTIC ASSAY FOR COMMERCIAL PURPOSES THAT IS FREE OF THE LIMITATIONS OF THE ORIGINAL CBA.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government