SBIR Phase I:Electronic allergy diagnostics: Photo-immobilization as a general strategy for attaching structurally and compositionally diverse ligands onto a single support

Award Information
National Science Foundation
Award Year:
Phase I
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
NSF 09-609
Small Business Information
9020 SW Washington Square Rd, Suite 450, Portland, OR, 97223
Hubzone Owned:
Socially and Economically Disadvantaged:
Woman Owned:
Principal Investigator
 Brian Clare
 (503) 626-1034
Business Contact
 Brian Clare
Title: PhD
Phone: (503) 626-1034
Research Institution
This Small Business Innovation Research (SBIR) Phase 1 project aims to rapidly address the feasibility of using photochemical linkers to immobilize small molecules, synthetic peptides, purified proteins and crude natural extracts onto an electrochemical sensor array. This study is motivated by 1) the need for multiplexed, low cost diagnostics for disease states in which appropriate capture ligands can vary broadly in structure and composition and 2) a need for general attachment methods that are insensitive to structural and compositional differences. Specifically, this project is aimed at diagnostics for drug, food and environmental allergies. We propose to explore the use of perfluorinated phenyl azide (PFPA) chemistry as a reactive and non-selective immobilization strategy for building these diverse arrays. This Phase 1 project aims to identify a single photochemical protocol for preparing arrays in which the immobilized materials retain biological activity. This method will enable the fabrication of highly multiplexed sensor arrays for use in point-of-care diagnostics. The broader impact/commercial potential of this project is to reduce the cost of allergy testing and enable physicians to make evidence-based decisions during a patient's initial office visit (<30 min). Current serological allergy diagnostics available in the market utilize a radioallergosorbent test (RAST) or an enzyme-linked immunosorbent assay (ELISA). These tests can be multiplexed, but require a central laboratory reader, a one week turn-around time and a cost of $15-20 per allergen. The combination of quantitative electrochemical detection with rapid, multiplexed diagnosis of 100+ biomarkers in a single serum sample will provide a significant improvement to existing technologies. If successful, it would be transformative in the clinical diagnosis of allergy diseases, enabling rapid evaluation at the doctor's office in a format that is significantly preferable to laboratory tests, skin-prick testing or food challenges. In addition to the commercial potential of allergy diagnostics, the Phase 1 SBIR project will develop and disseminate a general method for preparing diverse arrays of biologically-active materials on a single support. This new technology would have application in many other fields that affect our health, such as diagnostics for infectious disease and cancer and devices for the detection of food and environmental contaminants.

* information listed above is at the time of submission.

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