Biosensor to measure microscopic cell function

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$144,712.00
Award Year:
2006
Program:
STTR
Phase:
Phase I
Contract:
1R41GM070041-01A2
Award Id:
80350
Agency Tracking Number:
GM070041
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
CELLULAR ENGINEERING TECH, INC., 1112 ESTRON ST, IOWA CITY, IA, 52246
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ALAN MOY
(319) 887-2873
moya@celleng-tech.com
Business Contact:
ALAN MOY
(319) 335-4402
MOYA@CELLENG-TECH.COM
Research Institution:
IOWA STATE UNIVERSITY

IOWA STATE UNIVERSITY
1138 PEARSON HALL
AMES, IA, 50011

Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): This is a revised application. The broad, long-term objectives of this Phase I STTR proposal is to demonstrate feasibility in developing a novel optical-electronic biosensor for microscopic applications to permit simultaneous integration of photonic and electronic signals for comprehensive time-lapsed microscopy applications. Optical microscopy has limitations in sensitivity to evaluate the impact of signal transduction on cell-membrane function. Light microscopy is unable to detect cell-membrane displacement at the low nanometer level, and its specificity is limited by the potential over-interpretation of observed microscopic changes at the micron level. Thus, optical signals need to be properly interpreted within the contexts of an independent method. In contrast, electronic sensors have the resolution to measure cell surface cell membrane function at the low nanometer level but have limited intracellular spatial resolution. In collaboration with Iowa State University, CET Inc. will develop a novel, optical-electronic biosensor that will measure several functional parameters in cultured cells in a nondestructive fashion with a resolution of transmission electron microscopy. This sensor will combine the complementary strengths of electronic and optical signals. The outcome of this research is to demonstrate feasibility that will leverage a Phase II proposal that leads to further developments in sensor design, software and hardware development and image analysis and signal processing to provide comprehensive information on signal transduction in cultured living cells. The commercial product will provide a comprehensive platform for cell microscopists. The specific objectives for Phase I of this STTR are: (1) fabricate an optical-electronic sensor that can quantitatively and dynamically measure cell adhesion and motility without electrode signal degradation; (2) experimentally validate that the sensor is not inherently toxic to cells over both short and long time periods; (3) validate that the sensor does not impede the photonic signals from phase, DIC, and fluorescent microscopy.

* information listed above is at the time of submission.

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