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Wide field of view 3D microscopy for calcium imaging and photostimulation

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43MH111463-01
Agency Tracking Number: R43MH111463
Amount: $469,426.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 101
Solicitation Number: PA15-269
Solicitation Year: 2015
Award Year: 2016
Award Start Date (Proposal Award Date): 2016-08-10
Award End Date (Contract End Date): 2018-01-31
Small Business Information
Frederick, CO 80504-6412
United States
DUNS: 109079004
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (303) 833-4333
Business Contact
Phone: (303) 833-4333
Research Institution

Project Summary Abstract
Mental disease including schizophrenia depression and autism spectrum disorders are still poorly
understood although it is clear that they mostly represent cortical disorders The cortex is the primary site of
higher mental functions and despite extensive research there is still no unified theory of how the cortex works
This is partly due to the fact that neuroscientists have traditionally relied on microelectrodes to record the
activity of individual cells However cortical circuits are composed of millions of neurons and it is conceivable
that single cell measurements alone will not be sufficient to unravel function of the brain Optical imaging
techniques tackle this emergent level of neuronal circuit activity and enable to image the activity of neuronal
ensembles in vitro and in vivo while preserving single cell resolution something that brain imaging techniques
such as MRI or PET cannot do Moreover the development of genetically encoded photosensitive proteins
optogenetics and optochemical caged compounds offers the opportunity to not only image the activity of
many neurons but also to optically control them
Advances in optogenetics has led to the development of two photon microscopes combined with spatial
light modulators SLM that allow the customer to split the laser beam into a holographic pattern that can be
used to image activity or photoactivate neurons simultaneously in D Multiple commercially available
microscopes are now offered Additionally researchers are customizing their own microscopes as a low cost
alternative to purchasing new commercially available microscopes The Meadowlark Optics MLO SLM is
preferentially utilized due to the unprecedented response time a factor of faster than competing SLMs
enabling more complex studies of brain function However the limited resolution of the SLM leads to studies
over a smaller volume and low fill factor leads to optical losses To address these concerns MLO is proposing
the development of a high speed Hz to Hz high fill factor high resolution SLM To reduce
cost and time to market MLO proposes to utilize an existing backplane representing a total savings of $ M
and year of development Meadowlark will realize a prototype system within the Phase I and will utilize
internal funds to complete the product Project Narrative
Microscopy with spatial light modulators SLMs enables use of optical techniques to study neuronal circuit
activity to both monitor and manipulate the activity of neuronal ensembles in vitro and in vivo As a result of
successful worldwide research in this field suppliers of commercial two photon microscopes feel there is
extensive product potential As such six major microscope suppliers have either licensed or are negotiating
the patents to existing IP filed by Columbia University regarding use of photostimulation and adaptive optics in
microscopy The Meadowlark Optics SLM as a key component in these commercial microscopes used to
create the volume of foci for D scan less multi site programmable excitation Development of a high
resolution high speed Hz to Hz SLM will provide commercial suppliers with a second generation
microscope that matches the field of view that the SLM can excite to the field of view and resolution of
commercial microscopes Additionally the SLM can be sold as a stand alone product addressing a wide range
of markets

* Information listed above is at the time of submission. *

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