Development of a microfludic device for removal of glycerol from cultured red blood cells

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43HL130123-01A1
Agency Tracking Number: R43HL130123
Amount: $222,782.00
Phase: Phase I
Program: SBIR
Awards Year: 2016
Solicitation Year: 2017
Solicitation Topic Code: NHLBI
Solicitation Number: HL15-030
Small Business Information
1694 RED FOX DRIVE, Red Wing, MN, 55066-1468
DUNS: 078697905
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 BRIAN BISCHOFF
 (612) 802-0219
 brian.bischoff@hwinnovate.com
Business Contact
 BRIAN BISCHOFF
Phone: (612) 802-0219
Email: brian.bischoff@hwinnovate.com
Research Institution
N/A
Abstract
Abstract Recent advancements in the ability to produce blood products from expandable stem cells might be used to create a strategic reserve to reduce the strain on volunteer donors A strategic stem cell derived frozen supply may reduce seasonal shortages enhance the ability to respond to emergencies and address periodically inadequate supplies of rare blood types Unlike the donor based system where small numbers of units are collected in geographically distributed locations stem cell derived red blood cell products RBCs will be produced in large volumes at a central location and will be frozen to enable distribution and adequate shelf life In addition to improving the stem cell derived manufacturing process new economical ways of preparing the frozen RBCs for transfusion are needed The principal obstacle to the use of frozen blood has been the difficulty in removing cryoprotective agents glycerol post thaw to enable clinical use Red blood cell deglycerolization is typically time consuming requires expensive centrifuge based equipment and typically results in high cell losses Without an easy to use and economical method to wash frozen RBCs the practicality a of stem cell derived strategic supply will be greatly impaired The long term objective of this investigation is to develop a microfluidic device capable of removing glycerol from cryopreserved red blood cells This phase I investigation describes initial steps to take a prototype device developed in an academic laboratory with funding from National Institutes of Biomedical Imaging and Bioengineering NIBIB R EB and bring that invention toward commercialization The device developed will be easy to use largely automated requiring no external power and improve on cell loss Our central advancement is a vertically oriented microfluidic device which can be used to remove glycerol with minimal losses and minimal operator intervention Narrative The long term objective of this investigation is to develop a microfluidic device capable of removing glycerol from cryopreserved red blood cells This phase I investigation describes initial steps to take a prototype device developed in an academic laboratory with funding from National Institutes of Biomedical Imaging and Bioengineering NIBIB R EB and bring that invention toward commercialization The device developed will be easy to use largely automated requiring no external power and improve on cell loss Our central advancement is a vertically oriented microfluidic device which can be used to remove glycerol with minimal losses and minimal operator intervention

* Information listed above is at the time of submission. *

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