FEEDBACK CONTROLLED OLIGONUCLEOTIDE SYNTHESIZER PHASE I

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$221,047.00
Award Year:
1984
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
522
Agency Tracking Number:
522
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
4560 Horton St., Suite Q-214, Emeryville, CA, 94608
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Mickey S. Urdea
Principal Investigator
(415) 655-8729
Business Contact:
() -
Research Institute:
n/a
Abstract
THE INVESTIGATIONS HAVE PROPOSED THE DEVELOPMENT OF AN AUTOMATED OLIGONUCLEOTIDE SYNTHESIZER INCORPORATING A NUMBEROF NEW FEATURES. USING PHOSPHITE-TRIESTER METHODOLOGY FOR OLIGODEOXYRIBONUCLEOTIDE SYNTHESES OCCASIONALLY LOW YIELDS OCCASIONALLY LOW YIELDS ARE OBTAINED IN THE CONDENSATION STEP. THIS PROBLEM CAN BE OVERCOME BY MULTIPLE CONDENSATIONS. HOWEVER, NO ASSAYS ARE CURRENTLY AVAILABLE TO DETERMINE THE EXTENT OF COUPLING IMMEDIATELY FOLLOWING THIS STEP. THE INVESTIGATORS PROPOSE TO DEVELOP AN ANALYTICAL METHOD BASED ON THE COULOMETRIC GENERATION OF IODINE AND SUBSEQUENT STOICHIOMETRIC OXIDATION OF PHOSPHITESTO PHOSPHATES. ELECTROCHEMICAL GENERATION OF A PRECISE QUANTITY OF IODINE WITH ENDPOINT DETERMINATION BY A UV MONITOR WOULD PROVIDE FEEDBACK CONTROL THAT WOULD ALLOW CONDENSATION STEPS TO BE REPEATED WHEN NECESSARY. AUTOMATION AND FEEDBACK CONTROL WOULD BE ACHIEVED THROUGH INTERFACING A NEW TYPE OF REACTOR AND SOLVENT DELIVERY SYSTEM, WITH THE ELECTROCHEMICAL GENERATOR, AND DETECTION SYSTEM, VIA AN APPLE IIE COMPUTER. CONTINUED DEVELOPMENT OF THE REACTION SYSTEM IS PLANNED TO GIVE A UNIT WITH PARALLEL MULTISYNTHESIS CAPABILITIES. A SYSTEM SO CONFIGURED IS PROPOSED TO HAVE THE CAPABILITY OF SYNTHESIZING OLIGOMERS OF GREATER THAN 120 BASES. IMPROVEMENTS PLANNED FOR PHASE II, INCLUDE DEVELOPMENT OF A TUBE REACTOR AND PURIFICATION OF THE OLIGOMER PRODUCTS ON THE SOLID SUPPORT.

* information listed above is at the time of submission.

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