IONIZING RADIATION DISRUPTS CELLULAR ACTIVITIES BY CHEMICALLY DAMAGING PROTEIN AND DEOXYRIBONUCLEIC ACID (DNA) MOLECULES.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$48,688.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
3027
Agency Tracking Number:
3027
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Suite B, Arcadia, CA, 91006
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
RICHARD F. HAMMEN
PRINCIPAL INVESTIGATOR
(818) 574-7656
Business Contact:
() -
Research Institute:
n/a
Abstract
IONIZING RADIATION DISRUPTS CELLULAR ACTIVITIES BY CHEMICALLY DAMAGING PROTEIN AND DEOXYRIBONUCLEIC ACID (DNA) MOLECULES. ONE OF THE ABUNDANTLY FORMED CHEMICAL MODIFICATIONS OF DNA IS THYMIDINE DIOL (TD), FORMED BY THE ADDITION OF HYDROXYL RADICALS TO THYMIDINE. THE OBJECTIVE OF THIS PHASE I RESEARCH IS TO DEVELOP A MOLECULAR RADIATION DOSIMETER WHICH DIRECTLY MEASURES LOW-LEVEL RADIATION DAMAGE TO THE DNA OF EXPOSED TISSUES. THIS INTRACELLULAR MOLECULAR DOSIMETER WILL BE USED IN PHASE II TO EVALUATE THE EFFICACY OF RADIOPROTECTIVE DRUGS IN PREVENTING RADIATION INDUCED DAMAGE TO DNA. 60CO-GAMMA IRRADIATED DNA WILL BE ENZYMATICALLY HYDROLYSED TO NUCLEOSIDES. THE THYMIDINE DIOL WILL BE ISOLATED ON A HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY COLUMN. TO DETECT THIS MOLECULE, IT WILL BE DERIVATISED WITH FLUORESCEIN ISOTHIOCYANATE. THE RESULTING 5-THIOURETHANE WILL BE CHROMATOGRAPHICALLY ISOLATED FROM OTHER SUBSTANCES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND DETECTED BY FLUOROMETRY. THE SENSITIVITY OF THIS ASSAY IS EXPECTED TO DETECT ONE TD PER MILLION BASE PAIRS OF DNA.

* information listed above is at the time of submission.

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