Cryptosporidium Detection System

Award Information
Agency:
Environmental Protection Agency
Branch
n/a
Amount:
$70,000.00
Award Year:
2001
Program:
SBIR
Phase:
Phase I
Contract:
68-D-01-012
Award Id:
53370
Agency Tracking Number:
68-D-01-012
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
111 Roberts St, Suite K, East Hartford, CT, 06108
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
ArturoPilar
(860) 528-9737
fernandez@ciencia.com
Research Institute:
n/a
Abstract
The Phase I objective is to develop a compact, low-cost, easy-to-use system for the rapid detection of Cryptosporidium oocysts. Two key innovations provide the foundation for Ciencia, Inc.'s system: (1) a dielectrophoretic field-flow-fractionation method to replace current filtering and centrifugation methods to concentrate the sample and remove nontarget organisms or nonbiological debris, and (2) direct fluorescent immunoassay detection using a novel type of fluorescent label that should result in a sensitivity improvement of the order of 104 over that of conventional fluorescent immunoassays. Fluorescence excitation will be provided by single quantum well light-emitting diodes. This will result in a low-cost, compact instrument of very low power consumption. These innovations can be used to improve current laboratory-based procedures for Cryptosporidium detection, but more importantly, they can be exploited to implement portable devices for rapid onsite detection, and offer the potential of implementation of online sensors

* information listed above is at the time of submission.

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