Protein Microarrays for Bioreactor Bioproduct Monitoring

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$600,000.00
Award Year:
2002
Program:
SBIR
Phase:
Phase II
Contract:
NAS9-02079
Award Id:
56527
Agency Tracking Number:
012983
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
111 Roberts Street, Suite K, East Hartford, CT, 06108
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Salvador Fernandez
Principal Investigator
(860) 528-9737
fernandez@ciencia.com
Business Contact:
Arturo Pilar
Business Official
(860) 528-9737
apilar@ciencia.com
Research Institution:
n/a
Abstract
Rotating wall vessels (RWV) are widely used for cell culture in simulated microgravity conditions. Sensor technologies capable of monitoring the expression of proteins on a large scale (proteomics) are needed to fully exploit the unique capabilities of RWVs. This Phase II effort is part of a program to develop an advanced analytical monitoring system for quantitative detection of bioreactor bioproducts in microgravity. The underlying technology is grating-coupled surface plasmon resonance, which permits sensitive, label-free, parallel detection of bioproducts in a complex fluid without purification steps in real time. The objective of this effort is to develop generic capture microarrays that will allow for the site-directed self-assembly of tailor-made, user-defined high-density multi-component assay chips. This is accomplished by immobilizing a library of short oligonucleotide PNA (peptide nucleic acid) probe molecules on a chip in a site-specific manner using covalent attachment chemistry and a spotting method. Capture molecules that will compose the array are tagged (off-line) with a library of complementary PNA sequences. Then, the microarray is assembled by flowing the complex mixture of different PNA-tagged compounds over the chip, which through hybridization reactions, form site-directed self-assembled arrays. A set of microarrays will be produced and a complementary set of protein-PNA conjugates relevant to microgravity research.

* information listed above is at the time of submission.

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