SURFACE PLASMON RESONANCE PROTEIN ARRAY PHENOTYPING

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43DK061117-01
Agency Tracking Number: DK061117
Amount: $145,641.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
CIENCIA, INC., 111 ROBERTS ST, STE K, EAST HARTFORD, CT, 06108
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 SALVADOR FERNANDEZ
 (860) 528-9737
 FERNANDEZ@CIENCIA.COM
Business Contact
 ARTURO PILAR
Phone: (860) 528-9737
Email: APILAR@CIENCIA.COM
Research Institution
N/A
Abstract
A microarray-based system for label-free, high throughput protein expression in profiling in very small volumes of tissue or blood (approximately 10 mL) is proposed. The system will consist of an instrument, and a proteomic biosensor chip integrated into a fluidics cartridge. It utilizes a novel grating-coupled surface plasmon resonance (GC-SPR) imaging technology that will enable measurement of thousands of analytes simultaneously without the limitations of reporter molecules. We will use rapid assessment of cellular activation in cell cultures as a model system to develop and evaluate the GC-SPR chip- based technology. We expect that this technology will provide a new powerful tool for metabolic and physiologic phenotyping of laboratory animals as well as for investigating the metabolic outcome of genetic variation in humans. It will be applicable to the detection of a broad range of metabolic products, signaling molecules, hormones, enzymes, receptors and other proteins. Examples include rapid measurement of cytokine profiles, simultaneous measurement of many hormone concentrations in tissue samples, and assessment of intracellular signal transduction cascades. The technology is also suitable for minimally invasive microarrays in clinical settings where diagnostic profiles could be measured from a drop of blood instead of a large sample from a vein.

* Information listed above is at the time of submission. *

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