Development of an Ultrasound-Mediated Delivery System for the Mass Immunization of Fish

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$79,953.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
2002-33610-11953
Award Id:
57334
Agency Tracking Number:
2002-00349
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
P.O. Box 712, Buhl, ID, 83316
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Scott LaPatra
(208) 543-3456
scottl@clearsprings.com
Business Contact:
Randy MacMillan
Vice President of Research and Environme
(208) 543-3456
randy@clearsprings.com
Research Institute:
n/a
Abstract
Losses due to disease in world aquaculture are estimated to be in the billions of dollars annually. Vaccination is effective in preventing disease for many fish pathogens, however, lack of cost-effective methods for delivering vaccines prevents vaccination from becoming more prevalent in aquaculture. We have shown how ultrasound can enhance the uptake of both dissolved compounds and particles from the aquatic medium into fish, non-invasively. Many vaccines that are efficacious by injection do not provide protection when delivered by immersion, however, an ultrasound mediated delivery system could eliminate the need for injection. DNA vaccines have been shown to be very effective in salmon and trout and are a promising alternative to traditional antigen-based vaccines but require intramuscular injection. What we are proposing is to develop an ultrasound-mediated delivery protocol that would enable the mass administration of a vaccine making the process considerably cost-effective, as well as less stressful to the fish. The model for development of this system would be a DNA vaccine attached to biopolymer nanoparticles which we have shown can be used to successfully deliver DNA, including DNA vaccines. The efficacy of the delivery system for rainbow trout will be evaluated by determining gene expression, elicitation of a humoral immune response, and protection from lethal virus challenge.

* information listed above is at the time of submission.

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