THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING TUMOR NECROSIS FACTOR (TNF) AND, BY MANIPULATION OF THAT SEQUENCE, TO PRODUCE AND SECRETE THE MATURE TNF PROTEIN FROM THE YEAST SACCHAROMYCES CEREVISIAE.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3032
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1985
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
128 Spring Street, Lexington, MA, 02173
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 DONALD TAYLOR MOIR
 PRINCIPAL INVESTIGATOR
 (617) 861-9700
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING TUMOR NECROSIS FACTOR (TNF) AND, BY MANIPULATION OF THAT SEQUENCE, TO PRODUCE AND SECRETE THE MATURE TNF PROTEIN FROM THE YEAST SACCHAROMYCES CEREVISIAE. PREVIOUS WORK ON TNF HAS SHOWN IT TO HAVE CYTOSTATIC OR CYTOLYTIC ACTIVITY IN VITRO AGAINST A NUMBER OF TRANSFORMED CELL LINES AND TO HAVE TUMOR NECROTIC ACTIVITY AGAINST VARIOUS TUMORS IN ANIMAL MODELS. MOST IMPORTANTLY, IT HAS NO ADVERSE EFFECT ON NORMAL CELLS EITHER IN CULTURE OR IN ANIMAL MODELS. THIS SPECIFICITY FOR TUMOR CELLS SUGGESTS A POTENTIAL VALUABLE THERAPEUTIC ROLE FOR TNF IN TREATING HUMAN TUMORS. IN ORDER TO ESTABLISH ITS VALUE, HOWEVER, SUFFICIENT QUANTITIES OF THIS RATHER RARE PROTEIN MUST BE ISOLATED FOR TESTING. THE OVERALL AIM OF THIS RESEARCH PROPOSAL IS TO PRODUCE TNF ECONOMICALLY IN LARGE QUANTITIES FROM YEAST CELLS. AS A RESULT OF THE PHASE I EFFORTS DESCRIBED HERE, A CDNA COPY OF THE TNF GENE WILL BE ISOLATED AND CHARACTERIZED. IN PHASE II, THE PORTION OF THE GENE CODING FOR MATURE TNF WILL BE ATTACHED TO YEAST PROMOTER AND SECRETION SIGNAL SEQUENCES, AND THE ENTIRE TRANSCRIPTIONAL UNIT WILL BE INTEGRATED STABLY INTO THE YEAST GENOME. THE RESULT OF PHASE II SHOULD BE A GENETICALLY STABLE YEAST STRAIN SECRETING 1020 MG OF TNF PER LITER OF MEDIUM.

* Information listed above is at the time of submission. *

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