WE PROPOSE TO IDENTIFY THE GENES FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS BY THE USE OF RECOMBINANT DNA TECHNIQUES.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
2947
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Collaborative Research Inc
128 Spring Street, Lexington, MA, 02173
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DONALD WARREN BOWDEN
PRINCIPAL INVESTIGATOR
(617) 861-9700
Business Contact:
() -
Research Institution:
n/a
Abstract
WE PROPOSE TO IDENTIFY THE GENES FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS BY THE USE OF RECOMBINANT DNA TECHNIQUES. THE SYSTEM THAT WE ARE USING LENDS ITSELF TO HIGH-LEVEL EXPRESSION OF THESE ANTIGENS AS HYBRID PROTEINS IN E. COLI. THIS APPROACH FACILITATES PRODUCTION OF THESE ANTIGENS FOR THE PURPOSE OF ANTIGEN CHARACTERIZATION, GENERATION OF ANTIBODIES AND DEVELOPMENT OF IMMUNODIAGNOSTIC TESTS. A CDNA LIBRARY WILL BE CONSTRUCTED FROM HUMAN PANCREAS TISSUE. CLONING WILL BE INTO THE LAMBDA GT11 SYSTEM. CLONES CARRYING ISLET CELL ANTIGEN SEQUENCES WILL BE IDENTIFIED BY DIFFERENTIAL IMMUNOSCREENING WITH SERA FROM TYPE I DIABETES PATIENTS AND SERA FROM NORMAL INDIVIDUALS. WITH THE LAMBDA GT11 SYSTEM, CLONES CARRYING DIABETES-ASSOCIATED SEQUENCES CAN BE INDUCED TO EXPRESS SUBSTANTIAL AMOUNTS OF THE PANCREAS ANTIGEN FUSED TO BETA-GALACTOSIDASE (HYBRID PROTEIN). BECAUSE OF THEIR LARGE SIZE, THESE PROTEINS CAN SUBSEQUENTLY BE EASILY PURIFIED. THE GOAL OF THIS WORK IS TO CLONE, EXPRESS AND CHARACTERIZE ISLET CELL ANTIGEN GENES AND TO DEVELOP A SIMPLE TEST FOR ISLET CELL ANTIBODIES USING ANTIGENS PRODUCED BY RECOMBINANT DNA METHODS. THE USE OF RECOMBINANT DNA TECHNIQUES GIVES THIS METHODOLOGY THE POWER TO IDENTIFY SPECIFIC DISEASE-ASSOCIATED EPITOPES. THIS WILL FACILITATE THE DEVELOPMENT OF A

* information listed above is at the time of submission.

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