EXPERIENCE WITH THE CLONING AND EXPRESSION OF CLONED GENES IN SACCHAROMYCES CEREVISIAE SUGGESTS THAT THE RECOVERED YIELD OF CORRECTLY FOLDED PROTEIN PRODUCT IS GREATLY IMPROVED BY THE USE OF A YEAST-SECRETION PROCESS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4873
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Collaborative Research Inc
128 Spring Street, Lexington, MA, 02173
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ROBERT ANDREW SMITH
PRINCIPAL INVESTIGATOR
(617) 862-4130
Business Contact:
() -
Research Institution:
n/a
Abstract
EXPERIENCE WITH THE CLONING AND EXPRESSION OF CLONED GENES IN SACCHAROMYCES CEREVISIAE SUGGESTS THAT THE RECOVERED YIELD OF CORRECTLY FOLDED PROTEIN PRODUCT IS GREATLY IMPROVED BY THE USE OF A YEAST-SECRETION PROCESS. THIS IS ESPECIALLY TRUE FOR PROTEINS THAT ARE NORMALLY SECRETED FROMTHEIR NATURAL CELL SOURCES. THUS, COLLABORATIVE RESEARCH WILL FUSE THE GENE FOR HUMAN PROINSULIN TO A VARIETY OF YEAST PROMOTER AND SECRETION SIGNAL SEQUENCES AND EXPRESS ITIN YEAST IN AN ATTEMPT TO ISOLATE A STRAIN THAT SECRETES COMMERCIALLY VIABLE AMOUNTS OF PROINSULIN. A NUMBER OF MUTANT YEAST STRAINS ARE AVAILABLE THAT HAVE AN INCREASED CAPACITY FOR THE SECRETION OF FOREIGN PROTEINS. THESE SUPERSECRETING STRAINS WILL BE TESTED FOR THEIR EFFICACY IN SECRETING PROINSULIN. ONCE REASONABLE YIELDS OF SECRETED PROINSULIN ARE OBTAINED, THE PROINSULIN'S STRUCTURE WILL BE ANALYZED IN TERMS OF DISULFIDE BOND CONTENT, AMINO TERMINAL PROCESSING, AND CONVERTABILITY TO INSULIN.

* information listed above is at the time of submission.

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