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Development of high-throughput cardiotoxicity and hepatotoxicity assays with magnetic 3D bioprinting
Phone: (760) 448-1168
Email: htseng5@gmail.com
Phone: (832) 472-8128
Email: gsouza@n3dbio.com
PROJECT SUMMARYThe goal of this proposal is to develop assays for cardiotoxicity and hepatotoxicity using magneticD
bioprintingBoth cardiotoxicity and hepatotoxicity are critical areas for safety testingand in need of more
accurate in vitro screens to prevent costly market withdrawalsHowevercurrent in vitro options are lacking in
throughput and representation of native tissue environmentsThis proposal uses magneticD bioprinting to
createD in vitro culture models that mimic native tissueThe principle behind magneticD bioprinting is the
magnetization of cells and their printing using magnetic forcesThis method is rapid and easy to useand
escapes the technical issue of otherD culture models that limit its use for high throughput screeningIn Phase
Ia general toxicity assaythe BiO Assaywas developed using magneticallyD bioprinted spheroidsThis
Phase II builds on that successby developing this cytotoxic assay for cardiomyocytes and hepatocytesthen
further developing tissue specific functional assays to determine the effect of compounds on tissue functionFor
cardiomyocytesa beating assay will be developed to assay a compound s effect on beatingwhile for
hepatocytesan assay for cytochrome Pinduction inhibition will be developed to assay a compound s effect
on metabolismIn combination with other assayssuch as LC MS and fluorescent calcium signaling assaysour
assays will offer high content and high throughput solutions to study both cytotoxicity and functional toxicityThis work aids NIEHS in its goal of finding in vitro solutions for toxicity screening that are high throughput
and representative of native tissue environmentsThe end result of this proposed work is a full suite of assays to
study a compound s cardiotoxic and hepatotoxic effects
* Information listed above is at the time of submission. *