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Developing an automated yeast dissection system for aging research

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41AG058368-01
Agency Tracking Number: R41AG058368
Amount: $225,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: NIA
Solicitation Number: PA16-303
Solicitation Year: 2016
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-09-30
Award End Date (Contract End Date): 2018-09-29
Small Business Information
9350 KIRBY DR, STE 200, Houston, TX, 77054-2528
DUNS: 079349467
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (832) 538-1925
Business Contact
Phone: (832) 538-1925
Research Institution
HOUSTON, TX, 77030-3411
 Nonprofit college or university
Project Summary Developing an Automated Yeast Dissection System for Aging Research Aging is the single greatest risk factor for diseases that are principal causes of mortality The objectives of aging research are to discover key genes and pathways related to aging that may eventually contribute to retardation of aging and a delay in the onset of age associated diseases The budding yeast Saccharomyces cerevisiae has been a powerful model for the study of aging and has enabled significant contributions to our understanding of basic mechanisms of aging in eukaryotic cells However traditional assays of yeast aging including microdissection methods have technical challenges for instance the methods are low throughput and the experimental procedures are laborious An experiment typically lasts several weeks or months and requires overnight storage of the assayed cells at a refrigerator to pause replication throughout the course of experiment This tedious procedure has substantially hindered progress in the field of aging research We have developed an innovative and highly effective microfluidic platform for continuous and automatic dissection of daughter cells without disturbing mother cells as they bud The platform allows an automated whole lifespan tracking with high spatiotemporal resolution and large scale quantification of single yeast cells resulting in significant reduction of labor time and cost In addition the high resolution florescence imaging of yeast cells grown in constant and dynamically changing environments offers the ability to examine the dynamics of gene expression and signaling networks in a high throughput manner The quantity and types of data acquired by this platform are impossible with the traditional assay methods Herein we propose to develop automated dissection platform that enable fast and high throughput studies of yeast aging We will validate the reliability and capability of the proposed platform We propose to validate the prototype automated dissection platform for two important assays in yeast aging research determination of replicative lifespan for mutants in an automated and unattended fashion and continuous tracking the changes in abundance and cellular localization for specific GFP fusion proteins during the entire aging process Longer term plan of this proposal is to commercialize a fully automation benchtop system for high throughput studies of cellular aging Narrative Developing an Automated Yeast Dissection System for Aging Research In this proposal we seek to develop a commercial microfluidic platform to study yeast budding and lifespan in a revolutionary high throughput manner to provide a better understanding on aging The commercialized platform will offer an unparalleled method to significantly accelerate the discovery of novel conserved aging regulators and pathways by providing a fast high throughput and accurate analysis at the single cell level in budding yeast

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