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Integrated Platform for Mass Spectrometric Studies of Protein Structure

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM121133-01A1
Agency Tracking Number: R41GM121133
Amount: $210,175.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: 100
Solicitation Number: PA16-303
Timeline
Solicitation Year: 2016
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-09-01
Award End Date (Contract End Date): 2019-02-28
Small Business Information
1622 SAN CARLOS AVE, SUITE C
San Carlos, CA 94070-2060
United States
DUNS: 967100921
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 MARSHALL BERN
 (650) 637-0713
 bern@parc.com
Business Contact
 MARSHALL BERN
Phone: (650) 412-4210
Email: bern@proteinmetrics.com
Research Institution
 WASHINGTON UNIVERSITY
 
Campus Box 1054 1 Brookings Drive
SAINT LOUIS, MO 63130-4862
United States

 Nonprofit College or University
Abstract

Summary
Technology for studying the higher order structure HOS of biomolecules encompasses some
of the most important tools in all of science and is a highlighted NIGMS research topic Mass
spectrometric methods for studying HOS include oxidative footprinting and other methods to
covalently label amino acid side chain functional groups and also hydrogen deuterium
exchange HDX on protein backbone amides These methods require only small amounts of
not necessarily pure material and can study conformation and dynamics of proteins in solution
with or without ligands Mass spectrometric methods are especially well suited to biopharma
comparability and epitope mapping studies Currently there is no software that can analyze
mass spectrometric data from both chemical labeling and HDX experiments even though the
two methods both probe solvent accessibility and if combined would give finer resolution and
greater confidence Protein Metrics has already built beta test software for covalent labeling
including Fast Photochemical Oxidation of Proteins FPOP The proposed project will focus
on data analysis software for HDX experiments The outcome will be vendor neutral software
with advanced analytical and reporting capabilities that can handle both HDX and covalent
labeling experiments Narrative
The higher order structure HOS of biomolecules determines function binding partners
immune response and safety and efficacy of therapeutics Mass spectrometric
methods for studying HOS include hydrogen deuterium exchange and covalent labeling
experiments such as oxidative footprinting The proposed project will result in unique
software to analyze all types of mass spectrometric data for HOS studies and will find
use in both academic research and the biopharmaceutical industry

* Information listed above is at the time of submission. *

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