A novel fragmentation technique for tandem mass spectrometry

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43RR028158-01A1
Agency Tracking Number: RR028158
Amount: $218,473.00
Phase: Phase I
Program: SBIR
Awards Year: 2010
Solicitation Year: 2010
Solicitation Topic Code: NCRR
Solicitation Number: PHS2010-2
Small Business Information
DUNS: 123310083
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: Y
Principal Investigator
 () -
Business Contact
Phone: (443) 539-0139
Research Institution
DESCRIPTION (provided by applicant): The primary goal of this proposal is to develop a novel fragmentation technique based on interaction of peptide ions with radical beam. We plan to demonstrate that this technique will produce different type of fragment ions compared to those obtained in collision-induced dissociation. It will also overcome problems associated with collision-induced dissociation, e.g. preferential fragmentation and facile losses of labile groups involved in many important posttranslational modifications. Combining both fragmentation techniques in one high performance QqTOF mass spectrometer will substantially increase the value of these instruments for high throughput proteomics applications. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of the proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high throughput technology for increasing the efficiency of protein identification and structure elucidation using tandem mass spectrometry.

* Information listed above is at the time of submission. *

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