FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$521,000.00
Award Year:
1994
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
19066
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Covalent Associates Inc.
10 State Street, Woburn, MA, 01801
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Margaret Langmuir
(617) 938-1140
Business Contact:
() -
Research Institution:
n/a
Abstract
WE WILL SYNTHESIZE NEW PROBES FOR THE DETERMINATION OF INTRACELLULAR GLUTATHIONE AND OTHER CELLULAR THIOLS. THE REACTION PRODUCT OF THE PROBES WITH THIOLS WILL HAVE LONGER WAVELENGTH ABSORPTION AND EMISSION AND HIGHER QUANTUM YIELD THAN THE BEST CYTOSOLIC THIOL FLUOROPHORES CURRENTLY IN USE FOR CELL SORTING BY FLOW CYTOMETRY. THE DYES WILL HAVE THE ADVANTAGE OF BEING CELL MEMBRANE PERMEABLE SO THAT CELL LOADING CAN OCCUR AT LOWER EXTRA CELLULAR DYE CONCENTRATIONS THAN CURRENTLY POSSIBLE. THE SYNTHESIZED PROBES WILL BE TESTED IN VITRO AND IN CELL CULTURES FOR ABSORPTION AND EMISSION PROPERTIES WHEN BOUND TO GLUTATHIONE AND OTHER PROTEIN THIOLS. CELL TOXICITY WILL ALSO BE DETERMINED.

* information listed above is at the time of submission.

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