Sample Preparation Chip for PCR Detection of Water Borne Pathogens

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$80,000.00
Award Year:
2005
Program:
SBIR
Phase:
Phase I
Contract:
2005-33610-15595
Award Id:
72572
Agency Tracking Number:
2005-00192
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
11609 Lake Potomac Drive, Potomac, MD, 20854
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PeixuanZhu
(240) 453-6265
pzhu@creatvmicrotech.com
Business Contact:
PeixuanZhu
(240) 453-6265
pzhu@creatvmicrotech.com
Research Institute:
n/a
Abstract
Enterohemorrhagic Escherichia coli (E. coli O157:H7) is a causative agent of food- and water-borne diseases. Newly developed rapid immunoassay- and PCR-based techniques have been used for detection of E. coli O157:H7 in water and food samples. However, they have proven inadequate, because of the large sample size, an insufficient number of pathogens in the sample, sample splitting, antibody cross reaction, and PCR inhibition caused by sample contaminants. We propose a rapid, sensitive, specific and quantitative test for water-borne pathogens. The method can be employed wherever PCR is used to determine the presence, concentration and/or genetic characteristics of bacterial or viral pathogens. Greatest benefit will be seen in the analysis of samples that are large in relation to the typical 2-5 microliter template volume for PCR, particularly for water quality, food safety, and environment tests. We will develop the method initially to detect E. coli O157:H7 in drinking water in Phase I and source and recreational waters in Phase II. The public will benefit greatly from safer drinking water, rivers and beaches.

* information listed above is at the time of submission.

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