A bifunctional antibody screening system for both phage display and yeast two-hyb

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$200,035.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
1R43AI085700-01
Agency Tracking Number:
AI085700
Solicitation Year:
2010
Solicitation Topic Code:
NIAID
Solicitation Number:
PHS2010-2
Small Business Information
AFFOMIX, INC.
AFFOMIX, INC., 688 East Main Street, Branford, CT, 06405
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
623623803
Principal Investigator:
MICHAEL WEINER
(203) 458-2844
MICHAEL@MWEINER.COM
Business Contact:
WEINER
(203) 458-2844
mweiner@affomix.com
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): This is a proposal to establish a proof-of-principle for a vector system to isolate high-affinty antibodies through selective rounds in both yeast and Escherichia coli. Antibodies have quickly become extremely useful as therapeutic medicines to treat a wide-variety of disorders. We propose to develop a means of making the discovery of immunotherapeutics faster and cheaper. In this proposal we describe a system to isolate antigen-specific antibodies in yeast two-hybrid and then quickly evolve them for both higher affinity and specificity using phage display. This new vector system will eliminate the need for any subcloning needed for protein expression, Y2H and phage display. The option for expressing and purifying antibodies directly from E. coli or yeast is a direct application of the described system. The described system will allow for simultaneous testing of cDNA and antibody-antigen interactions in both yeast and E.coi-based systems. Completion of this project will form the basis of a scalable system for a rapid and inexpensive means of affinity-maturation of recombinant antibodies screened in Y2H and increasing our understanding of protein- protein interactions. PUBLIC HEALTH RELEVANCE: Researchers will benefit from an increased understanding of the function of human proteins. Methods are needed that can facilitate this understanding. The immediate objective of our research is to generate a method that will enable researchers to multiplex the screening of affinity reagents. We anticipate using our high-throughput pipeline for producing recombinant antibodies as a means to generate the reagents needed to make this method possible.

* information listed above is at the time of submission.

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