Point-of-Care Diagnostic for Acute Q Fever using LAMP

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$730,000.00
Award Year:
2010
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-09-C-0036
Agency Tracking Number:
A2-4000
Solicitation Year:
2008
Solicitation Topic Code:
A08-178
Solicitation Number:
2008.3
Small Business Information
Agave BioSystems, Inc.
P.O. Box 100, Ithaca, NY, -
Hubzone Owned:
Y
Socially and Economically Disadvantaged:
Y
Woman Owned:
N
Duns:
022552900
Principal Investigator:
Janet Huie
Staff Scientist
(607) 272-0002
jhuie@agavebio.com
Business Contact:
Noe Salazar
President
(512) 656-6200
nsalazar@agavebio.com
Research Institution:
n/a
Abstract
Q fever, caused by the bacterium Coxiella burnetii, presents with acute symptoms indistinguishable from many other blood infections. Chronic infection, more frequently developing in immunocompromised individuals, presents a high mortality rate (up to 60% of chronic cases) and often requires open-heart surgery to repair substantial valve damage. Q fever can be airborne or tick-borne and is a significant detriment in deployment conditions. It is important to diagnose Q-fever during its acute phase to direct appropriate treatment and identify personnel requiring follow-up monitoring for chronic disease. While serology tests have been commercialized for Q fever detection, it is necessary to test both IgG and IgM against two types of C. burnetii antigens to differentiate between acute and chronic disease stages. Additionally, a high false positive rate occurs for these assays, and the antibody response is difficult to detect early in infection. To eliminate these shortcomings, Agave BioSystems proposed to integrate a loop-mediated isothermal amplification (LAMP) reaction built into a point-of-care field diagnostic device. To date, we have been highly successful with the implementation of a breadboard device for real time readings and expect to continue the development of a hand-held, fully-enclosed device, capable of providing early detection of acute phase Q-Fever.

* information listed above is at the time of submission.

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