TOXIC CELLULAR DNA DAMAGE AND DISEASES

Award Information
Agency: Department of Defense
Branch: Army
Contract: N/A
Agency Tracking Number: 3814
Amount: $49,910.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Cytec Inc
Po Box 57462, Webster, TX, 77598
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 DR TOD S JOHNSON
 (713) 484-4610
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS PHASE I SBIR PROJECT IS TO STUDY TECHNICAL/ METHODOLOGICAL ASPECTS, PERFORMANCE CRITERIA FOR ANEW CLINICAL SPECIMEN TOXICITY TEST. STATE-OF-THE-ART FLOW CYTOMETRIC/MONOCLONAL ANTIBODY TECHNOLOGY WOULD BE USED TO DETECT DNA DAMAGE, CYTOKINETIC ABNORMALITIES, AND ANEUPLOIDY. GENOTOXICITY AND CYTOKINETIC ABNORMALITIES WOULD BE ASSESSED IN VITRO BY LABELING WITH A HALOGENERATED PHRIMIDINE (IDURD) AND THEN USING MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT AND THE AMOUNT OF IDURD INCORPORATED (DNA REPLICATION PATTERN) VIA A FLUORESCENT TAGGED MONOCLONAL ANTIBODY HIGHLY SPECIFIC FOR IDURD. WE PROPOSE TO DEVELOP: 1) AN IMMUNOTOXICITY TEST BASED ON SHORT-TERM CULTURE, BLASTOGENEIC RESPONSE TO PHA MITOGEN STIMULATED, PERIPHERAL BLOOD LYMPHOCYTES (ULTIMATELY T-& BCELL SUBSETS); AND 2) A HEPATOTOXICITY TEST USING IN VITRO IDURD LABELING OF FINE NEEDLE ASPIRATION BIOPSIES. PHASE I STUDIES WOULD UTILIZE DOMESTIC CANINES, WHEREAS BOTH CANINE AND HUMAN CLINICAL MATERIAL WOULD BE STUDIED IN PHASE II. THE HIGH TECHNOLOGY TEST(S) TO BE DEVELOPED WOULD BE VERSATILE; HAVE BROAD COMMERCIAL AND MILITARY APPLICATIONS; AND REPRESENT A MAJOR STEP FORWARD ANALYTICALLY FOR TOXICITY TEST DETECTION OF DNA DAMAGE, CELLULAR ABNORMALITIES, AND DISEASES ACQUIRED NATURALLY OR BY EXPOSURE TO BIOLOGICAL WEAPONS.

* information listed above is at the time of submission.

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