DEVELOPMENT OF A STOPPED-FLOW CYTOMETER

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$100,000.00
Award Year:
1990
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
7488
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Cyto Fluidics Inc.
Eng Rsch Ctr Bldg 334, University Of Mayland, College Park, MD, 20742
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
James F Brown
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
THE PROPOSED SBIR PHASE I EFFORT IS TO DESIGN, DEVELOP, AND EVALUATE A STOPPED-FLOW SORTING CYTOMETER. THIS SYSTEM WILLBE BASED ON CYTO FLUIDICS' PATENTED "FLUID TRANSISTOR" WHICH ALLOWS MICROFABRICATION OF FLUID CONTROL VALVES IN SIZES AND DENSITIES AT THE LIMITS OF CURRENT LITHOGRAPHIC METHODS (2 UM). IN THE PROPOSED STUDY, CFI WILL TEST THE FEASIBILITY OF UTILIZING THIS NEW TECHNOLOGY TO CREATE "ON CHIP" SYSTEMS OF CAPILLARY CHANNELS AND HIGH-SPEED MICROVALVES TO CONTROL THE FLOW (POSITION) OF PARTICLES AND CELLS. THE PRIMARY AIMS OF PHASE I WILL BE: (1) TO DECREASE VALVE CYCLE TIMES SO AS TO INCREASE THE CELL SORTING RATE, (2) TO OPTIMIZE THE GEOMETRY OF THE MICRO-MACHINED FLOW CHANNELS TO MINIMIZE TURBULENCE AND CELL STRESS, AND (3) TO EVALUATE THE STOPPED-FLOW SORTING SYSTEM USING PARTICLES AND CELLS. USING DESIGN CRITERIA ESTABLISHED IN PHASE I, THE PHASE II OBJECTIVE WOULD BE TO CREATE AN INEXPENSIVE FLOW CYTOMETER. CFI'S UNIQUE TECHNOLOGY COULD MAKE POSSIBLE THE IMPLEMENTATION OF FUNCTIONS SUCH AS THE REAL-TIME INTRODUCTION OF REAGENTS INTO THE SINGLE CELL ENVIRONMENT, SECONDARY SORTING, HIGH SPEED ANALYSIS USING PARALLEL FLOW CHANNELS, AND THE COMBINATION OF FLOW CYTOMETRY AND HIGH RESOLUTION IMAGE ANALYSIS.

* information listed above is at the time of submission.

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