THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP TECHNIQUES FOR USING MONOCLONAL ANTIBODIES LABELED WITH 212BISMUTH, AN ALPHA PARTICLE-EMITTING RADIONUCLIDE, AS IN VITRO OT IN VIVOCYTOTOXIC AGENTS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4736
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Cytogen Corpon
201 College Road East, Princeton Forrestal Center, Princeton, NJ, 08540
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 A DWIGHT LOPES
 PRINCIPAL INVESTIGATOR
 (609) 452-8838
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP TECHNIQUES FOR USING MONOCLONAL ANTIBODIES LABELED WITH 212BISMUTH, AN ALPHA PARTICLE-EMITTING RADIONUCLIDE, AS IN VITRO OT IN VIVOCYTOTOXIC AGENTS. ANTIBODY CONJUGATES WILL BE DEVELOPED FORRADIOIMAGING TO LOCALIZE TO THE TUMOR SITE ONLY, WITH MINIMAL UPTAKE BY THE RETICULOENDOTHELIAL SYSTEM, MAKING THEM IDEAL AGENTS FOR IMMUNOTHERAPY. THIS SITE-SPECIFIC COVALENT ATTACHMENT OF CHELATORS TO THE OLIGOSACCHARIDES IN THE CONSTANT REGION, AWAY FROM THE ANTIBODY'S BINDING SITE, YIELDS HOMOGENEOUS CONJUGATES THAT RETAIN THEIR ANTIGEN BINDING ACTIVITY AND AFFINITY. THESE CHEMICAL TECHNIQUES ARE AN IMPROVEMENT OVER EXISTING METHODS FOR ANTIBODY MODIFICATION. 212BISMUTH HAS BEEN CHELATED BY DTPA COVALENTLY ATTACHED TO MONOCLONAL ANTITUMOR ANTIBODIES BY THE ABOVE METHODS. IN THIS PHASE I PROJECT, THE RESEARCH WILL DEMONSTRATE IMMUNOSPECIFIC KILLING OF TUMOR CELLS IN VITRO AND STABILITY OF THE MAB-DTPA-212BI CONJUGATE IN VIVO.IN PHASE II, RADIOIMMUNOTHERAPY MAY BE ACHIEVED IN AN IN VIVO MURINE TUMOR MODEL. ANTIBODY LOCALIZATION WILL BE STUDIED AND THERAPEUTIC EFFECTS ASSESSED. THE ULTIMATE GOALOF RADIOIMMUNOTHERAPY IS THE TREATMENT OF HUMANS FOR CANCER.

* information listed above is at the time of submission.

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