THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP TECHNIQUES FOR USING MONOCLONAL ANTIBODIES LABELED WITH 212BISMUTH, AN ALPHA PARTICLE-EMITTING RADIONUCLIDE, AS IN VITRO OT IN VIVOCYTOTOXIC AGENTS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4736
Agency Tracking Number:
4736
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
201 College Road East, Princeton Forrestal Center, Princeton, NJ, 08540
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
A DWIGHT LOPES
PRINCIPAL INVESTIGATOR
(609) 452-8838
Business Contact:
() -
Research Institute:
n/a
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP TECHNIQUES FOR USING MONOCLONAL ANTIBODIES LABELED WITH 212BISMUTH, AN ALPHA PARTICLE-EMITTING RADIONUCLIDE, AS IN VITRO OT IN VIVOCYTOTOXIC AGENTS. ANTIBODY CONJUGATES WILL BE DEVELOPED FORRADIOIMAGING TO LOCALIZE TO THE TUMOR SITE ONLY, WITH MINIMAL UPTAKE BY THE RETICULOENDOTHELIAL SYSTEM, MAKING THEM IDEAL AGENTS FOR IMMUNOTHERAPY. THIS SITE-SPECIFIC COVALENT ATTACHMENT OF CHELATORS TO THE OLIGOSACCHARIDES IN THE CONSTANT REGION, AWAY FROM THE ANTIBODY'S BINDING SITE, YIELDS HOMOGENEOUS CONJUGATES THAT RETAIN THEIR ANTIGEN BINDING ACTIVITY AND AFFINITY. THESE CHEMICAL TECHNIQUES ARE AN IMPROVEMENT OVER EXISTING METHODS FOR ANTIBODY MODIFICATION. 212BISMUTH HAS BEEN CHELATED BY DTPA COVALENTLY ATTACHED TO MONOCLONAL ANTITUMOR ANTIBODIES BY THE ABOVE METHODS. IN THIS PHASE I PROJECT, THE RESEARCH WILL DEMONSTRATE IMMUNOSPECIFIC KILLING OF TUMOR CELLS IN VITRO AND STABILITY OF THE MAB-DTPA-212BI CONJUGATE IN VIVO.IN PHASE II, RADIOIMMUNOTHERAPY MAY BE ACHIEVED IN AN IN VIVO MURINE TUMOR MODEL. ANTIBODY LOCALIZATION WILL BE STUDIED AND THERAPEUTIC EFFECTS ASSESSED. THE ULTIMATE GOALOF RADIOIMMUNOTHERAPY IS THE TREATMENT OF HUMANS FOR CANCER.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government