THIS PROJECT WILL DEVELOP A SYSTEM FOR THE REMOVAL OF T LYMPHOCYTES OR TUMOR CELLS FROM BONE MARROW USING IMMOBILIZED MONOCLONAL ANTIBODIES.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4735
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Cytogen Corpon
210 College Road East, Princeton Forrestal Center, Princeton, NJ, 08540
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
RICHARD C SEIGEL
PRINCIPAL INVESTIGATOR
(609) 987-8200
Business Contact:
() -
Research Institution:
n/a
Abstract
THIS PROJECT WILL DEVELOP A SYSTEM FOR THE REMOVAL OF T LYMPHOCYTES OR TUMOR CELLS FROM BONE MARROW USING IMMOBILIZED MONOCLONAL ANTIBODIES. THE SELECTIVE REMOVAL OFT CELLS FROM BONE MARROW USING IMMOBILIZED MONOCLONAL ANTIBODIES WILL PROVIDE AN EFFECTIVE SYSTEM FOR ALLOGENIC TRANSPLANT OF BONE MARROW IN A VARIETY OF DISEASE STATES. THIS SYSTEM WILL ALSO SERVE AS A MODEL FOR TUMOR REMOVAL IN AUTOLOGOUS BONE MARROW TRANSPLANT. PREVIOUS WORK HAS DEMONSTRATED THE FEASIBILITY OF SELECTIVELY REMOVING T CELL SUBPOPULATIONS FROM FLOWING, CITRATED, WHOLE HUMAN BLOOD. THIS PHASE I RESEARCH WILL QUANTITATE THE CHARACTERISTICS OFA CONTINUOUS FLOW DEVICE THAT WILL REMOVE LEUKEMIC CELLS FROM BLOOD. THE CAPACITY OF THIS SYSTEM, AND ITS DEPLETION KINETICS AND IMMUNOLOGIC SPECIFICITY, WILL BE DETERMINED. THE ANTIBODIES WILL BE SITE-SPECIFICALLY IMMOBILIZED USING PROPRIETARY CHEMISTRY; SITE-SPECIFIC IMMOBILIZATION CHEMISTRY INVOLVES OXIDATION OF THE OLIGOSACCHARIDE MOIETIESOF THE ANTIBODIES THAT ARE LOCATED IN THE CONSTANT REGION OFTHE ANTIBODY HEAVY CHAIN. COUPLING THE SUBSTRATE TO THE OLIGOSACCHARIDE SHOULD RESULT IN IMMUNOREACTIVE SURFACES WITH EXCELLENT RETENTION OF IMMUNOSPECIFICITY, THEREBY REPRESENTING AN IMPROVED METHOD FOR SEPARATING THE DESIRED CELL POPULATIONS.

* information listed above is at the time of submission.

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