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Genetically Enhanced Nitrogen Fixation with Algae Biofilms

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2017-33610-26720
Agency Tracking Number: 2017-00408
Amount: $99,733.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 8.7
Solicitation Number: N/A
Timeline
Solicitation Year: 2017
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-07-01
Award End Date (Contract End Date): 2018-07-31
Small Business Information
615 S ARAPEEN DR STE 310
Salt Lake City, UT 84108-1254
United States
DUNS: 078584999
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 John Watkins
 Principal Investigator
 (801) 792-0652
 drjohnjwatkins@comcast.net
Business Contact
 John Watkins
Title: CEO
Phone: (801) 792-0652
Email: drjohnjwatkins@comcast.net
Research Institution
N/A
Abstract

This project will develop an algae-base nitrogen fixation process for commercial ammonia production for the agronomy market. This process operates under standard conditions. The technology utilizes a whole-cell mutant algae strain grown on an electrode surface and takes advantage of enzymatic pathways to make ammonia at standard conditions. The primary inputs are electricity and water. Compared to the current Haber-Bosch process, the proposed technology has less capital costs, generates ammonia at a lower cost and permits distributed on-demand production of fertilizer on a local scale, enabling many smaller fertilizer plants to be built at lower cost and bridge the gap in availability. The objectives of this project are increase the production rate to 25 ┬Ámole ammonia per cm2 per hour through genetic engineering, Increase the scale of the project by an order of magnitude, 50 cm2 electrode to 500 cm2. The algae mutant expresses nitrogenase through a high population of heterocyst cells. Genetic engineering at the University of Utah will be used to increase the expression of the nitrogenase enzyme in the cell itself. The scale up of the process will require a new cell to be constructed and an examination of the process conditions. The incorporation of opaque electrodes and maintaining enough light for the algae is seen as a unique challenge. The proposed method will be the first continuous process, where the enzymes are stimulated for increased production and the product is expressed extracellularly.

* Information listed above is at the time of submission. *

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