Tunable Source for Protein Fluorescence Lifetime Studies

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$175,301.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
1R43RR018439-01A1
Award Id:
70767
Agency Tracking Number:
RR018439
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2201-A 12TH STREET NORTH, FARGO, ND, 58102
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
GREGORY GILLISPIE
(701) 237-4908
GILLISPIE@DAKOTATECHNOLOGIES.COM
Business Contact:
GREGORY GILLISPIE
(701) 237-4908
GILLISPIE@DAKOTATECHNOLOGIES.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): Dakota Technologies, Inc. (DTI) has developed and demonstrated a low-cost and user-friendly method that generates fluorescence decay curves with exceptional speed, accuracy, and precision. Sample fluorescence is induced by a Nd:YAG microchip laser that operates at high repetition rate (5-10 kHz) and delivers sub-nanosecond pulses. Waveforms of the resulting fluorescence decay are directly recorded via a photomultiplier tube (PMT) and fast digitizer. The available excitation wavelengths are limited to the Nd:YAG harmonics at 532, 355, and 266 nm. Excitation sources that can be continuously tuned over a broad wavelength range and retain the high pulse repetition frequency and short pulse duration will be developed during this Phase I SBIR project. Particular emphasis will be placed on the 275-315 nm range that is optimal for fluorescence characterization of proteins. By perfecting these sources and integrating them into a commercial spectrofluorimeter, DTI will provide biochemists, biophysicists, and molecular biologists with a versatile, high performance tool for cutting edge research. A promising industrial application, verifying that proteins are produced in the appropriate conformation, has also been identified.

* information listed above is at the time of submission.

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