Fluorescence Lifetime-Based Kinase Assay for HTS

Award Information
Agency:
Department of Health and Human Services
Amount:
$14,526.00
Program:
SBIR
Contract:
1R43GM074338-01A1
Solitcitation Year:
2006
Solicitation Number:
PHS2006-2
Branch:
N/A
Award Year:
2006
Phase:
Phase I
Agency Tracking Number:
GM074338
Solicitation Topic Code:
N/A
Small Business Information
DAKOTA TECHNOLOGIES, INC.
2201-A 12TH STREET NORTH, FARGO, ND, 58102
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 KIRK HARTEL
 (701) 237-4908
 KHARTEL@DAKOTATECHNOLOGIES.COM
Business Contact
 ROXANE MEIDINGER
Phone: (701) 237-4908
Email: RMEDINGER@DAKOTATECHNOLOGIES.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): Fluorescence based-assays have greatly facilitated high-throughput screening (HTS) in drug discovery. However, in doing so, they created a bottleneck relating to false positives and false negatives, which may arise due to interferences from light scattering, inner filter effects, and compound fluorescence. Unlike fluorescence intensity, a fluorescence lifetime-based measurement is resistant to light scattering and inner filter effects, and provides the selectivity needed to discriminate the target fluorescence signal from background fluorescence. In Phase I we will demonstrate a homogenous fluorescence lifetime-based kinase assay that is resistant to fluorescence interferences and does not require the use of antibodies. Overcoming fluorescence interferences will cut costs associated with secondary screening and assist quicker identification of lead compounds. Furthermore, it will create an opportunity to screen compound libraries and/or natural products that are known to have some fluorescent properties, but were previously inaccessible by means of traditional fluorescence-based methods. Kinases are a major class of drug targets, which are involved in human diseases such as cancer, diabetes, and arthritis. This technology will advance the speed and efficiency in which we can find drugs to treat these important diseases.

* information listed above is at the time of submission.

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