MAXIMUM LIKELIHOOD HIGH PERFORMANCE LIQUID CHROMATOGRAPHY MS/MS PROTEIN SEQUENCING

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 17305
Amount: $49,085.00
Phase: Phase I
Program: SBIR
Awards Year: 1992
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Daniel H. Wagner Associates
894 Ross Dr, Suite 205, Sunnyvale, CA, 94089
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Robert J. Lipshutz
 (408) 745-1800
Business Contact
Phone: () -
Research Institution
N/A
Abstract
MANY PROBLEMS IN DISEASE REMEDIATION, CELL BIOLOGY, EVOLUTION, AND GENETICS REQUIRE COMPLETE KNOWLEDGE OF THE SEQUENCE OF AMINO ACIDS WHICH CONSTITUTE A PARTICULAR PROTEIN. EXISTING METHODS OF SEQUENCING SUCH AS EDMAN DEGRADATIONS REQUIRE SIGNIFICANT AMOUNTS OF PROTEIN MATERIAL AND LONG PERIODS OF TIME. USING CAPILLARY HIGH PERFORMANCE LIQUID CHORMATOGRAPHY (HPLC) AND TANDEM MASS SPECTROMETRY (MS/MS), RESEARCHERS HAVE DEMONSTRATED THE ABILITY TO SEQUENCE FEMTOMOLE QUANTITIES OF A SINGLE PROTEIN. ONE LIMITATION OF THIS TECHNIQUE IS THE PROBLEM OFRECONSTRUCTING THE PROTEIN SEQUENCE FROM THE MASS SPECTRUM. WE PROPOSE TO DEVELOP A MAXIMUM LIKELIHOOD RECONSTRUCTION ALGORITHM TO SUPPORT HPLC MS/MS PROTEIN SEQUENCING.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government