NUCLEIC ACID HYBRIDIZATION (NAH), AN EXTREMELY IMPORTANT MOLECULAR BIOLOGY RESEARCH TOOL, HAS THE POTENTIAL TO BE A MAJOR DIAGNOSTIC PROCEDURE.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4737
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Digene
Building 34, University Of Maryland, College Park, MD, 20742
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 FLOYD TAUB
 PRINCIPAL INVESTIGATOR
 (301) 454-5906
Business Contact
Phone: () -
Research Institution
N/A
Abstract
NUCLEIC ACID HYBRIDIZATION (NAH), AN EXTREMELY IMPORTANT MOLECULAR BIOLOGY RESEARCH TOOL, HAS THE POTENTIAL TO BE A MAJOR DIAGNOSTIC PROCEDURE. HOWEVER, THE CURRENT TECHNIQUE REQUIRES THE USE OF EXPENSIVE, HAZARDOUS, AND TIME-CONSUMINGMETHODS BASED ON RADIOACTIVE MATERIALS. FURTHERMORE, NAH TESTS TYPICALLY RESULT IN EITHER NON-QUANTITATIVE OR ONLY SEMIQUANTITATIVE INFORMATION, A SERIOUS LIMITATION WHEN IT IS IMPORTANT TO KNOW IF AN ABNORMAL QUANTITY OF A SEQUENCE (E.G., ONCOGENES) IS PRESENT. A NEW NONISOTOPIC LABELING PROCEDURE WHICH TAKES LESS THAN 1 HOUR, REQUIRES LESS TOXIC AND LESS EXPENSIVE REAGENTS, APPEARS MORE RELIABLE, AND HAS A SENSITIVITY THAT IS HIGHER THAN PREVIOUS METHODS HAS BEEN IMPLEMENTED IN PRELIMINARY EXPERIMENTS. AN IMPROVED METHOD FOR QUANTIFYING RADIOACTIVE AND NONISOTOPIC HYBRIDIZATIONS BASED UPON COMPUTERIZED IMAGE PROCESSING HAS ALSO BEEN DEVELOPED. A PHASE I GRANT WILL PERMIT DIGENE TO: (1) TESTTHIS LABELING TECHNIQUE ON NUCLEIC ACIDS FROM TRANSFORMED CELLS TO DETERMINE THE FEASIBILITY OF USING THIS TECHNIQUE IN CLINICAL MEDICINE; AND (2) QUANTIFY THE EXTENT OF HYBRIDIZATIONS USING A PROTOTYPE IMAGE PROCESSING SYSTEM. IN PHASE II, THESE SYSTEMS WILL BE USED FOR THE ANALYSIS ANDQUANTITATION OF ONCOGENES, POTENTIALLY ONCOGENIC VIRUSES, AND CHEMOTHERAPY RESISTANCE IN A VARIETY OF CELL LINES AND CLINICAL SPECIMANS.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government