PRODUCTION OF URSODIOL FROM POULTRY BILE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16536
Amount: $47,030.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1991
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
51 Bissell Road, Lebanon, NJ, 08833
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Sipos, Tibor
 Principal Investigator
 (215) 758-5715
Business Contact
 1 R43 DK44047-01
Phone: () -
Research Institution
N/A
Abstract
THE OVERALL GOAL OF THIS PROJECT IS TO COMMERCIALLY PRODUCE URSODEOXYCHOLIC ACID (URSODIOL) FROM WASTE PRODUCTS OF THE POULTRY INDUSTRY BY EITHER A ONE-STEP MICROBIAL TRANSFORMATION PROCESS OR BY A CHEMICAL EPIMERIZATION OF CHENDIOL TO URSODIOL. URSODIOL IS MEDICALLY USED TO DISSOLVE GALLSTONES. URSODIOL IS VERY EXPENSIVE ($800 TO $2,000/KG) AND ONLY PRODUCED BY COMPANIES OUTSIDE THE U. S. A.. THE OBJECTIVE OF THIS PHASE I GRANT IS TO DEMONSTRATE THE TECHNICAL FEASIBILITY OF CONVERTING THE CHENODEOXYCHOLIC ACID (CHENODIOL) TO URSODIOL IN > 80% YIELDS. THE OBJECTIVE OF SUBSEQUENT PHASE II WORK WILL BE TO SCALE-UP THE MOST VIABLE PROCESS VIA PILOT-PLANT TO COMMERCIAL QUANTITIES. THE SPECIFIC AIMS OF THE PHASE I GRANT ARE TO: 1) SCREEN KNOWN AEROBIC AND ANAEROBIC BACTERIA FOR THEIR ABILITY TO CONVERT CHENODIOL TO URSODIOL. 2) CONCURRENTLY, DETERMINE THE FEASIBILITY OF CONVERTING CHENODIOL TO URSODIOL BY AN IMPROVED CHEMICAL EPIMERIZATION PATHWAY. 3) IDENTIFY OPTIMAL CONDITIONS SUCH AS PH, COFACTORS, METAL IONS, ETC. FOR THE BIOTRANSFORMATION OF CHENODIOL TO URSODIOL. 4) ISOLATE URSODIOL FROM THE CULTURE MEDIA BY FILTRATION THROUGH A CHROMATOGRAPHY COLUMN AND RECOVER URSODIOL FROM THE COLUMN BY A SOLVENT WASH. DETERMINE EFFICIENCY OF THE MICROBIAL VS. THE CHEMICAL PROCESS.

* Information listed above is at the time of submission. *

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